Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP002416	1,894,762	G→T	L67I (CTT→ATT)	Clo1313_1622 ←	peptidase M42 family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP002416	1,894,762	0	G	T	100.0%	102.9 / NA	41	L67I (CTT→ATT)	Clo1313_1622	peptidase M42 family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (18/23);  total (18/23)

TTGCGCCTATAATGCCATATATCTCTTTCTTTCCATGAATTACAACTTCCTGGGCCAGAAGGACCTTGCTGTCCACACCCCCAATGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAAGATGGGCTGTAACCATAATCCTTCTGCCTCCGGAACCGCCTATGCCCTTTTTTATCCCGATAACATTGTAGAATTCATCTATTTCCACACTGTCACAGTATTTTTCAAACAGCTTTGCAATGTACCCGGAAAGCTTGTCTTCC  >  CP002416/1894615‑1894904                                                                                                                                                    |                                                                                                                                               ttGCGCCTATAATGCCATATATCTCTTTCTTTCCATGAATTACAACTTCCTGGGCCAGAAGGACCTTGCTGTCCACACCCCCAATGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAATATg                                                                                                                                             <  1:231220/151‑1 (MQ=255)  tGCGCCTATAATGCCATATATCTCTTTCTTTCCATGAATTACAACTTCCTGGGCCAGAAGGAACTTGCTGTCCACACCCCCAATGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAATATgg                                                                                                                                            <  1:215649/151‑1 (MQ=255)   gcgcCTATAATGCCATATATCTCTTTCTTTACATGAATTACAACTTCCTGGGCCAGAAGGACCTTGCTGTCCACACCCCCAATGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAATATggg                                                                                                                                           <  1:1707049/151‑1 (MQ=255)    cgcCTATAATGCCATATATCTCTTTCTTTCCATGAATTACAACTTCCTGGGCCAGAAGGACCTTGCTGTCCACACCCCCAATGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAATATGGGc                                                                                                                                          <  1:1489294/151‑1 (MQ=255)    cgcCTATAATGCCATATATCTCTTTCTTTCCATGAATTACAACTGCCTGGGCCAGAAGGACCTTGCTGTCCACACCCCCAATGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAATATGGGc                                                                                                                                          <  1:1016289/151‑1 (MQ=255)                  tatatCGCTTTTATTCCAAGAATTACAACTTGATGTGCCAGAAGGCCCATGCTGTCCACACCCCCAATGTTTGAGAAAGTGATAAACCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAATATGGGCTGTAACCATAATcc                                                                                                                            <  1:1491310/151‑1 (MQ=255)                        tctttcttTCCATGAATTACAACTTCCTGGGCCAGAAGGCCCTTGCTGACCACCCCCCCAATGTTTGAGACAGTGATAATCCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAATATGGGCTGTAACCAAAATCCATCTGc                                                                                                                      >  1:920199/1‑151 (MQ=255)                           ttctgtcCATGAATTCCACCTTCCTGGGCCAGCAGGACCTTGCTGTCCACCCCCCCAATCTGTGAGACAGGGATAAAACCCTTTTAGTCAACACTTTTTACCCTCAAACCTATTACGTCAATATGGGCTGTAACCATAATCCTTCTGCCTc                                                                                                                   <  1:123775/151‑1 (MQ=255)                             ctttcCCTGAATTACAACTTCCTGGGCCAGAAGGACCTTGCTGTCCACACCCCCAATGTTTGAGACAGTGATAAACCCATTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAATTTGGGCGGTAACCATAATCCTTCTGCCTCCg                                                                                                                 <  1:920200/151‑1 (MQ=255)                              tttcCATGAATTACAACTTCCTGGGCCAGAAGGACCTTGCTGTCCACACACCCAATGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCgg                                                                                                                <  1:1472341/151‑1 (MQ=255)                                 cgatctaTTACAACTTCCTGGGCCAGAAGGACCTTGCTGTCCACACCCCCAATGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCGGAAc                                                                                                             <  1:1011146/145‑1 (MQ=255)                                  cATGAATTACAACTTCCTGGGCCAGAAGGACCTTGCTGTCCACACCCCCAATGTTTGAGACAGTGATAAACCCCTGTTCGTCAATACTTTTTACCATCAAACCGATTTCGTCAATATGGGCTGTAACCAGAATCCTTCTGCCTCCGGaccc                                                                                                            >  1:40029/1‑148 (MQ=255)                                  cATGAATTACAACTTCCGGGGCCAGAAGGACCTTGCTGTCCACACCCCCAATGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCGGAAcc                                                                                                            <  1:309383/151‑1 (MQ=255)                                       aTTACAACTTCCTGGGCCAGAAGGACCTTGCTGTCCACACCCCCAATGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTAAATATGGGCTGTAACCATAATCATTCTGCCTCCGGAAcagatcg                                                                                                       >  1:1011147/1‑145 (MQ=255)                                        ttACAACTTCCTGGGCCAGAAGGACCTTGCTGTCCACACCACCTATGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCGGAACCGCCTAt                                                                                                      <  1:1673707/151‑1 (MQ=255)                                                tccggggCCAGAAGGACCTTGCTGTCCACACCCCCAATGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACTTTTTACCATCAAACCTCTTTCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCGGAACCGCCTATGCCCtttt                                                                                              <  1:40030/147‑1 (MQ=255)                                                      gCCAGAAGGACCTTGCTGTCCACACCCCCAATGTTTGAGACAGTGATAAACCCCTTTTCGACAATACTATTTACCATCAAACCTATTTCGTCAATATGGGCTGTAACCATAAACCTACTGCCTCCGGAACCGCCTATGCCCGTTTTTATcc                                                                                        >  1:947973/1‑151 (MQ=255)                                                       cccgAAGGACCTTGCGGTCCACACCCCCAATGGTTGAGACAGTGAAAAACCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCGGAACCGCCTATGCCCTTTTTTATccc                                                                                       <  1:1861130/148‑1 (MQ=255)                                                              gACCTTGCTGTCCACACCCCCAATGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAATATGGGCGGTAACCATAATCCTTCTGCCTCCGGAACCGCCTATGCCCTTTTTTATCCCGATAACa                                                                                >  1:1605239/1‑151 (MQ=255)                                                               cccTTGCGGTCCACACCCCCAATGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCGGAACCGCCTATGCCCTTTTTTATCCCGATaagat                                                                               <  1:947974/150‑4 (MQ=255)                                                                ccTTGCTGTCCACACCCCCAATGTTTGAGACAGTGATACACCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCCGAACCGCCTATGCCCTTTTTTATACCGATAACAtt                                                                              >  1:1245321/1‑151 (MQ=255)                                                                ccTTGCTGTCCACACCCCCAATGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACTTTTTACCATCAAACATAATACGTCAATATGGGCTGAAACCATAATCCTTCTGACTCCGGAACCGCCAATGCCCTATATTATCCCGAAAACaaa                                                                              >  1:1220332/1‑149 (MQ=255)                                                                ccTTGCTGTCCACACACCCAATGTTTGAGACAGTGATAACCCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCGGAACCGCCTATGCCGGTTTTTATCCCGAGAACAtt                                                                              >  1:1627862/1‑151 (MQ=255)                                                                       gTCCACACCCACAATGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACGTTTTACCATAACACATAATTAGTGCATATGGGCCGTGACCATAATCCTTCTGCCTCCGGCCCCGCCGATGCCCGTTTTTATTCCGGTAACAttttataat                                                                       >  1:1067518/1‑144 (MQ=255)                                                                         ccACACCCCCAATGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACATTTTACCATCAAACCTATTTCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCGGCACCGCCTATGCCCGTTTTTATCCCGATATCAGTGTAGAATTc                                                                     >  1:82835/1‑151 (MQ=255)                                                                              cacccAATGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACTATTTACCAACAAACCTATGTCGTCAATATGGGCTGTAACCTAAAACCTTCTGCCTCCGGAACCGCCTATGCCCTTTTTTATACCGATAACATTGTTGAATTCaaaaa                                                                >  1:1834223/3‑147 (MQ=255)                                                                                   aaTGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCGGACCCGCCTATGCCCTTTTTTATCCCGATAACATTGTAGAATTCATCTATTTcc                                                           >  1:1147226/1‑151 (MQ=255)                                                                                         tGAGACAGTGATAAACCCCATTTCCTCAATACTTTTTACCATCAAACCTATTTCGTCAATATGCGCTGTAACCATAATCCTTCTGCCTCCGGAACCGCCTATGCCCGTATTTATCCCGATAACAATGTACAATTCATCTATATCCACACTg                                                     >  1:1705122/1‑151 (MQ=255)                                                                                                              ttCGTCAATACTTTTTACCATCAAACCTATTTCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCGGAACCGCCTATGCCCTTTTTTATCCCGATAACATTGTAGAATTCATCTATTTCCACACTGTCACAGTATTTTTCAAACAGc                                >  1:271032/1‑151 (MQ=255)                                                                                                              cttccgatctcTTTTTACCATCAAACCTATTTCGTCAATATGGGCTGTACCCATAATCCTTCTGCCTCCGGAACCGCCTATGACCATTTTTATCCCGATAACATTGTAGAATTCATCTATTTCCAAACTGTCACAGTATTTTTCAAACAGc                                <  1:1059457/141‑1 (MQ=255)                                                                                                                  tCAATACTATTTACCATCAAACCTATTTCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCGGAACCGCCTATGCCCTTTTTTATCCCGATAACATTGTAGAATTCATCTATTTCCACACTGTCACAGTATTTTTCAAACAGCTTTg                            <  1:1220331/151‑1 (MQ=255)                                                                                                                       aCTTTTTACCATCAAACCTATTTCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCGGAACCGACTATGCCCGTTTTTATCCCGATAACAATGTAGAATTCTTCTATTTCCACACTGTCACAGTATTTTTCAAACAGCTTTGCAATg                       >  1:1897623/1‑151 (MQ=255)                                                                                                                          ttttACCATCAAACCTCTTTCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCGGAACCGCCTATGCCCTTTTTTATCCCGATAACATTGTAGAATTCATCTATTTCCACACTGTCACAGTATTTTTCAAACAGCTTTGCAATGTAc                    <  1:795103/151‑1 (MQ=255)                                                                                                                           tttACCATCAAACCTATTTCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCGGAACCGCCTATGCCCTTTTTTATCCCGATAACATTGTAGAATTCATATATTTCCACACTGTCACACTATTTTTCAAACAGCTTTGCAATGTAca                   >  1:795104/1‑150 (MQ=255)                                                                                                                               ccATCAACCCTATTTCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCGGAACCGCCTCTGCCCTTTTTTATCCCGATAACATTGTAGAATTCATCTTTTTCCACACTGTCACAGTATTTTTCAACAAGCTTTGCAATGTACCCGGa               >  1:821113/1‑151 (MQ=255)                                                                                                                                      aCCTATTTCGTCAATATGTGCTGTAACCATAATCCTTCTGCCTCCGGAACCGCCTATGCCCTTTTTTATCCCGATAACATTGTAGAATTCATCTATTTCCACACTGTCACAGTATTTTTCAAACAGCTTTGCAATGTACCCGGAAAGCTTg        <  1:821114/151‑1 (MQ=255)                                                                                                                                        cTATTTCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCGGAACCGCCTATGCCCTTTTTTATCCCGATAACATTGTAGAATTCATCTATTTCCACACTGTCACAGTATTTTTCAAACAGCTTTGCAATGTACCCGGAAAGCTTGTc      >  1:1758859/1‑151 (MQ=255)                                                                                                                                        cTATTTCGTCAATATGGGCTGTAACCATAATCCTTCTGCCGCCGGAACCGCCTATGCCCTTTTTTATCCCGATAACATTGTAGAATTCATCTATTTCCACACTGTCACAGTATTTTTCAAACAGCTTTGCAATGTACCCGGAAAGCTTGTc      <  1:1189007/151‑1 (MQ=255)                                                                                                                                         tATTTCTTCAATATGGGCTGTAACCATAATCCTTGATCATCAGGAACAGACTATGCTCTTTTTTATCGCGATAACATTGTAGAATTCATCTATTTCCACACTGTATCAGTATTTTTCAAACAGCTTTGCAATGTACCCGGAAAGCTTGTCt     <  1:1629980/151‑1 (MQ=255)                                                                                                                                            ttCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCGGAACCGCCTATGCCCTTTTTTATCCCGATAACATTGTAGAATTCATCTATTTCCACACTGTCACAGTATTTTTCAAACAGCTTTGCAATGTACCCGGAAAGCTTGTCTTcc  <  1:1605240/151‑1 (MQ=255)                                                                                                                                            ttCGTCAATATGGGCTGTAACCATAATCCTTCTGCCTCCGGAACCGCCTATGCCCTTTTTTATCCCGATAACATTGTAGAATTCATCTATTTCCACACTGTCACAGTATTTTTCAAACAGCTTTGCAATGTACCCGGAAAGCTTGTCTTcc  <  1:1147225/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                               TTGCGCCTATAATGCCATATATCTCTTTCTTTCCATGAATTACAACTTCCTGGGCCAGAAGGACCTTGCTGTCCACACCCCCAATGTTTGAGACAGTGATAAACCCCTTTTCGTCAATACTTTTTACCATCAAACCTATTTCGTCAAGATGGGCTGTAACCATAATCCTTCTGCCTCCGGAACCGCCTATGCCCTTTTTTATCCCGATAACATTGTAGAATTCATCTATTTCCACACTGTCACAGTATTTTTCAAACAGCTTTGCAATGTACCCGGAAAGCTTGTCTTCC  >  CP002416/1894615‑1894904

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑