BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP002416	1,828,328	(G)_6→7	coding (793/1107 nt)	Clo1313_1569 ←	type IV pilus assembly protein PilM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP002416	1,828,328	1	.	G	92.7%	338.6 / 17.3	96	coding (793/1107 nt)	Clo1313_1569	type IV pilus assembly protein PilM
Reads supporting (aligned to +/- strand): ref base . (3/4); new base G (46/43); total (49/47)
Fisher's exact test for biased strand distribution p-value = 7.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

Only 100 of 106 total aligned reads displayed.

TATAATAAAAATCTTTGAAATCGGAGTACCATAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑G‑AGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAGCACTCTGTCTTTAAGAAAATTAACAATTGTC  >  CP002416/1828179‑1828476

tataATAAAAATCTTTGAAATCGGAGTACCATAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTgg‑ggg‑g                                                                                                                                                       >  1:2177166/1‑150 (MQ=255)

      aaaaaTCTTTGAAATCGGAGTACCATAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGGTGGG‑G‑AGTCa                                                                                                                                                 >  1:2177168/1‑150 (MQ=255)

       aaaaTCTTTGAAATCGGAGTACCATAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCAt                                                                                                                                                >  1:2177342/1‑150 (MQ=255)

       aaaaTCTTTGAAATCGGAGTACCATAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCAt                                                                                                                                                <  1:2177417/150‑1 (MQ=255)

        aaaTCTTTGAAATCGGAGTACCATAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGGCG‑AGTCAtt                                                                                                                                               >  1:2202143/1‑150 (MQ=255)

        aaaTCTTTGAAATCGGAGTACCATAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCAtt                                                                                                                                               >  1:2176833/1‑150 (MQ=255)

        aaaTCTTTGAAATCGGAGTACCATAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCAtt                                                                                                                                               <  1:2177354/150‑1 (MQ=255)

         aaTCTTTGAAATCGGAGTACCATAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTc                                                                                                                                              <  1:2177350/150‑1 (MQ=255)

         aaTCTTTGAAATCGGAGTACCATAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTc                                                                                                                                              <  1:2177352/150‑1 (MQ=255)

           tCTTTGAAATCGGAGTACCATAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCa                                                                                                                                            <  1:2177495/150‑1 (MQ=255)

            cTTTGAAATCGGAGTACCATAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCat                                                                                                                                           <  1:2177295/150‑1 (MQ=255)

             tttGAAATCGGAGTACCATAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCata                                                                                                                                          <  1:2177348/150‑1 (MQ=255)

             tttGAAATCGGAGTACCATAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCata                                                                                                                                          >  1:2202163/1‑150 (MQ=255)

                 aaaTCGGAGTACCATAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGtt                                                                                                                                      <  1:2177346/150‑1 (MQ=255)

                 aaaTCGGAGTACCATAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGtt                                                                                                                                      >  1:2177344/1‑150 (MQ=255)

                       gAGTACCATAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCGTTCAATAAAATTGCTGACGTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGAAAATCCATATGTTTTCTTa                                                                                                                                <  1:2177257/150‑1 (MQ=255)

                             cATAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTGTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGGTG‑AGTCATTCCATATGTTTTCTTAAGTcgt                                                                                                                          <  1:2177395/150‑3 (MQ=255)

                              aTAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCttt                                                                                                                         <  1:2177144/150‑1 (MQ=255)

                               tAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTc                                                                                                                        >  1:2177110/1‑150 (MQ=255)

                                aaCACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCa                                                                                                                       <  1:2177237/150‑1 (MQ=255)

                                   acCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTCAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑G‑AGTCATTCCATATGTTTTCTTAAGTCTTACAGCtt                                                                                                                   >  1:2177366/1‑150 (MQ=255)

                                   acCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCt                                                                                                                    <  1:2177142/150‑1 (MQ=255)

                                    ccTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCtt                                                                                                                   >  1:2177112/1‑150 (MQ=255)

                                    ccTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCtt                                                                                                                   >  1:2176971/1‑150 (MQ=255)

                                    ccTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCtt                                                                                                                   <  1:2177299/150‑1 (MQ=255)

                                      tttCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTcc                                                                                                                 >  1:2177114/1‑150 (MQ=255)

                                      tttCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTcc                                                                                                                 <  1:2177140/150‑1 (MQ=255)

                                      tttCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTca                                                                                                                 <  1:2177283/150‑2 (MQ=255)

                                      tttCAAGATAAAATTCAAAAAACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAAATGCTGACTATTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCGTAAGGCTATCAGCTTcc                                                                                                                 <  1:2177369/150‑1 (MQ=255)

                                       ttCAAGATAAAATTCAAAACGCTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCt                                                                                                                <  1:2177138/150‑1 (MQ=255)

                                         cAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGa                                                                                                              <  1:2177136/150‑1 (MQ=255)

                                               aaaaTTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACtt                                                                                                        >  1:2177101/1‑150 (MQ=255)

                                                 aaTTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTtat                                                                                                      <  1:2177261/150‑1 (MQ=255)

                                                  aTTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTtata                                                                                                     >  1:2177057/1‑150 (MQ=255)

                                                   ttCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTatat                                                                                                    >  1:2177103/1‑150 (MQ=255)

                                                     cAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTg                                                                                                  <  1:2177134/150‑1 (MQ=255)

                                                     cAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTg                                                                                                  <  1:2177132/150‑1 (MQ=255)

                                                        aaCACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGt                                                                                               >  1:2177118/1‑150 (MQ=255)

                                                        aaCACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGt                                                                                               >  1:2177116/1‑150 (MQ=255)

                                                        aaCACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGt                                                                                               >  1:2202119/1‑150 (MQ=255)

                                                        aaCACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGt                                                                                               <  1:2177130/150‑1 (MQ=255)

                                                          cacTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTc                                                                                             >  1:2177120/1‑150 (MQ=255)

                                                           acTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTcc                                                                                            >  1:2177122/1‑150 (MQ=255)

                                                             tttGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCt                                                                                          <  1:2132841/150‑1 (MQ=255)

                                                             tttGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCt                                                                                          >  1:2202151/1‑150 (MQ=255)

                                                               tGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTg                                                                                        <  1:2133105/150‑1 (MQ=255)

                                                               tGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTg                                                                                        <  1:2177126/150‑1 (MQ=255)

                                                                 cTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCa                                                                                      <  1:2177249/150‑1 (MQ=255)

                                                                 cTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCa                                                                                      >  1:2177124/1‑150 (MQ=255)

                                                                   aTCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAat                                                                                    >  1:2202153/1‑150 (MQ=255)

                                                                   aTCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGATTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAat                                                                                    >  1:2176839/1‑150 (MQ=255)

                                                                    tCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAata                                                                                   <  1:2176974/150‑1 (MQ=255)

                                                                         cGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTc                                                                              >  1:2176953/1‑150 (MQ=255)

                                                                          gggTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCg                                                                             >  1:2176949/1‑150 (MQ=255)

                                                                          gggTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCg                                                                             >  1:2176978/1‑150 (MQ=255)

                                                                            gTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTc                                                                           >  1:2202133/1‑150 (MQ=255)

                                                                             tAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCa                                                                          >  1:2176980/1‑150 (MQ=255)

                                                                              aaGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCaa                                                                         <  1:2177259/150‑1 (MQ=255)

                                                                                  cTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATAtt                                                                     >  1:2177059/1‑150 (MQ=255)

                                                                                  cTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATAtt                                                                     <  1:2177149/150‑1 (MQ=255)

                                                                                    ttCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTgg                                                                   >  1:2176982/1‑150 (MQ=255)

                                                                                      cAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGaa                                                                 <  1:2177267/150‑1 (MQ=255)

                                                                                       aaTAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑G‑AGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACt                                                               <  1:2177367/150‑1 (MQ=255)

                                                                                         tAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACtt                                                              >  1:2177047/1‑150 (MQ=255)

                                                                                         tAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACtt                                                              <  1:2177251/150‑1 (MQ=255)

                                                                                         tAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACtt                                                              >  1:2176984/1‑150 (MQ=255)

                                                                                            aaTTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCg                                                           >  1:2177109/1‑150 (MQ=255)

                                                                                                 cTGACTTTTCCGTAAGTTATTACATGTTCCCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGTAACTTCCGGAAAg                                                      >  1:2176986/1‑150 (MQ=255)

                                                                                                  tGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGa                                                     >  1:2176990/1‑150 (MQ=255)

                                                                                                  tGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGa                                                     >  1:2176988/1‑150 (MQ=255)

                                                                                                      ttttCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATaa                                                 >  1:2176992/1‑150 (MQ=255)

                                                                                                      ttttCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATaa                                                 >  1:2176994/1‑150 (MQ=255)

                                                                                                       tttCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAAc                                                >  1:2176845/1‑150 (MQ=255)

                                                                                                        ttCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACt                                               >  1:2202141/1‑150 (MQ=255)

                                                                                                             tAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGtt                                          <  1:2176972/150‑1 (MQ=255)

                                                                                                              aaGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTa                                         <  1:2177273/150‑1 (MQ=255)

                                                                                                               aGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTaa                                        <  1:2176970/150‑1 (MQ=255)

                                                                                                                  tattaCATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAAtt                                     >  1:2176996/1‑150 (MQ=255)

                                                                                                                  tattaCATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAAtt                                     >  1:2176998/1‑150 (MQ=255)

                                                                                                                   attaCATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTc                                    <  1:2177161/150‑1 (MQ=255)

                                                                                                                   attaCATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTc                                    >  1:2177000/1‑150 (MQ=255)

                                                                                                                     taCATGTTCTCTTATTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGATTAACTTTGTTAAATTCaa                                  <  1:2176968/150‑1 (MQ=255)

                                                                                                                      aCATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAg                                 <  1:2177151/150‑1 (MQ=255)

                                                                                                                          gTTCTCTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAGCAct                             >  1:2177097/1‑150 (MQ=255)

                                                                                                                            tctcTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAGCActct                           <  1:2176964/150‑1 (MQ=255)

                                                                                                                            tctcTTTTTGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAGCActct                           <  1:2176966/150‑1 (MQ=255)

                                                                                                                                    tGAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAGCACTCTGTCTTTaa                   <  1:2177345/150‑1 (MQ=255)

                                                                                                                                     gAAAGATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAGCACTCTGTCTTTAAg                  <  1:2176962/150‑1 (MQ=255)

                                                                                                                                        aGATTGTTGG‑GGGAG‑AGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAGCACTCTGTCTTTAAGaaa               <  1:2176960/150‑1 (MQ=255)

                                                                                                                                         gATTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAGCACTCTGTCTTTAAGaaaa              >  1:2176829/1‑150 (MQ=255)

                                                                                                                                          aTTGTTGG‑GGG‑GGAGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAGCACTCTGTCTTTAAGAAAAt             >  1:2177002/1‑150 (MQ=255)

                                                                                                                                           tgttggg‑ggg‑g‑AGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAGCACTCTGTCTTTAAGAAAATTa           >  1:2202147/6‑150 (MQ=255)

                                                                                                                                            tgttgGTGGG‑G‑AGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAGCACTCTGTCTTTAAGAAAATTa           <  1:2177169/150‑1 (MQ=255)

                                                                                                                                              tggg‑ggg‑g‑AGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAGCACTCTGTCTTTAAGAAAATTAACa        >  1:2202139/3‑150 (MQ=255)

                                                                                                                                               ggg‑ggg‑g‑AGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAGCACTCTGTCTTTAAGAAAATTAACaa       >  1:2177004/2‑150 (MQ=255)

                                                                                                                                               ggg‑ggg‑g‑AGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAGCACTCTGTCTTTAAGAAAATTAACaa       <  1:2177343/149‑1 (MQ=255)

                                                                                                                                                gg‑ggg‑g‑AGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAGCACTCTGTCTTTAAGAAAATTAACAAt      >  1:2202117/1‑150 (MQ=255)

                                                                                                                                                    gg‑g‑AGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAGCACTCTGTCTTTAAGAAAATTAACAATTgt   <  1:2177115/150‑1 (MQ=255)

                                                                                                                                                     g‑g‑AGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAGCACTCTGTCTTTAAGAAGATTAACAATTgtc  >  1:2177008/1‑150 (MQ=255)

                                                                                                                                                     g‑g‑AGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAGCACTCTGTCTTTAAGAAAATTAACAATTgtc  >  1:2177006/1‑150 (MQ=255)

TATAATAAAAATCTTTGAAATCGGAGTACCATAACACCTTTCAAGATAAAATTCAAAACACTTTGCTATCTGCCGGGTAAGCCTTTCAATAAAATTGCTGACTTTTCCGTAAGTTATTACATGTTCTCTTTTTGAAAGATTGTTGG‑GGG‑G‑AGTCATTCCATATGTTTTCTTAAGTCTTTCAGCTTCCTGAAGACTTATATTGAGTTCCCTTGCAATATGCTCGTCAATATTGGAACTTCCGGAAAGAATAACTTTGTTAAATTCAAGCACTCTGTCTTTAAGAAAATTAACAATTGTC  >  CP002416/1828179‑1828476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`