Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP002416	730,420	G→A	intergenic (+130/‑37)	Clo1313_R0024 → / → Clo1313_0644	tRNA‑Tyr/transposase IS200‑family protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP002416	730,420	0	G	A	100.0%	165.6 / NA	50	intergenic (+130/‑37)	Clo1313_R0024/Clo1313_0644	tRNA‑Tyr/transposase IS200‑family protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (31/19);  total (31/19)

TCCATCTCCCTCCACCAGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGGTTCGCCAACTACATTAAAAACAAAGGAGGTATCACGATGCGTGATAATGCT  >  CP002416/730274‑730471                                                                                                                                                   |                                                    tCCATCTCCCTCCACCAGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTc                                                  <  1:13774/150‑1 (MQ=255)    aTCTCCCTCCACCAGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGcc                                               <  1:13776/150‑1 (MQ=255)     tctcCCTCCACCAGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCa                                              >  1:11231/1‑150 (MQ=255)     tctcCCTCCACCAGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGGGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCa                                              >  1:18316/1‑150 (MQ=255)        ccctccACCAGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACt                                           <  1:16426/150‑1 (MQ=255)        ccctccACCAGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACt                                           <  1:13798/150‑1 (MQ=255)         cctccACCAGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTa                                          >  1:19254/1‑150 (MQ=255)          ctccACCAGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTAc                                         >  1:19204/1‑150 (MQ=255)           tccACCAGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACa                                        >  1:18122/1‑150 (MQ=255)           tccACCAGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACa                                        <  1:11137/150‑1 (MQ=255)            ccaccaGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACAt                                       <  1:11135/150‑1 (MQ=255)             caccaGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACAtt                                      >  1:10814/1‑150 (MQ=255)             caccaGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACAtt                                      <  1:11133/150‑1 (MQ=255)             caccaGACAAAAGCGAGTAAAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACAtt                                      >  1:11271/1‑150 (MQ=255)              accaGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTa                                     <  1:13804/150‑1 (MQ=255)                caGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTaaa                                   >  1:11279/1‑150 (MQ=255)                 aGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTaaaa                                  <  1:17292/150‑1 (MQ=255)                 aGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTaaaa                                  <  1:11127/150‑1 (MQ=255)                 aGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTaaaa                                  >  1:11281/1‑150 (MQ=255)                 aGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTaaaa                                  >  1:11283/1‑150 (MQ=255)                     aaaaGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACaa                              >  1:11303/1‑150 (MQ=255)                     aaaaGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACaa                              >  1:19170/1‑150 (MQ=255)                     aaaaGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACaa                              >  1:11305/1‑150 (MQ=255)                       aaGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAag                            >  1:19202/1‑150 (MQ=255)                       aaGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAag                            <  1:11109/150‑1 (MQ=255)                        aGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAagg                           >  1:12514/1‑150 (MQ=255)                        aGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAagg                           >  1:19232/1‑150 (MQ=255)                          cGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAaggag                         >  1:17814/1‑150 (MQ=255)                             gTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTa                      >  1:19262/1‑150 (MQ=255)                              tataAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTAt                     >  1:18324/1‑150 (MQ=255)                               ataAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTATc                    >  1:12374/1‑150 (MQ=255)                               ataAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTATc                    >  1:18048/1‑150 (MQ=255)                               ataAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTATc                    >  1:18420/1‑150 (MQ=255)                                taAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTATCa                   <  1:13788/150‑1 (MQ=255)                                taAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTATCa                   <  1:15886/150‑1 (MQ=255)                                  aaaTACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTATCACg                 <  1:13800/150‑1 (MQ=255)                                  aaaTACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTATCACg                 >  1:12426/1‑150 (MQ=255)                                  aaaTACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTATCACg                 >  1:11347/1‑150 (MQ=255)                                     tACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTATCACGATg              <  1:11061/150‑1 (MQ=255)                                        tCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTATCACGATGCGt           <  1:11047/150‑1 (MQ=255)                                         cGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTATCACGATGCGTg          <  1:16998/150‑1 (MQ=38)                                           cTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTATCACGATGCGTGAt        <  1:13784/150‑1 (MQ=25)                                            ttttttGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTATCACGATGCGTGATa       <  1:17066/150‑1 (MQ=12)                                            ttttGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGTGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTATCACGATGCGTGATa       >  1:14564/1‑150 (MQ=255)                                            ttttGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTAAATTAAAAACAAAGGAGGTATCACGATGCGTGATa       >  1:12538/1‑150 (MQ=255)                                              ttGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTATCACGATGCGTGATAAt     >  1:18282/1‑150 (MQ=18)                                               tgtgTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTATCACGATGCGTGATAATg    >  1:18392/1‑150 (MQ=17)                                               tgtgTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTATCACGATGCGTGATAATg    >  1:11391/1‑150 (MQ=17)                                                 tgtTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTATCACGATGCGTGATAATGCt  >  1:11405/1‑150 (MQ=14)                                                 tgtTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGATTCGCCAACTACATTAAAAACAAAGGAGGTATCACGATGCGTGATAATGCt  >  1:11401/1‑150 (MQ=14)                                                                                                                                                   |                                                    TCCATCTCCCTCCACCAGACAAAAGCGAGTATAAAATACTCGCTTTTGTGTTTTTGTTTAAAGAAAACCACTGGCTATGCCAGTGGTTCTAAAGAGACAGTGTCTATGAGTAGAAAAAAGTAACCTCCTTTGTTACAATAATGTAGGTTCGCCAACTACATTAAAAACAAAGGAGGTATCACGATGCGTGATAATGCT  >  CP002416/730274‑730471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑