Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0024161,620,2970TG27.4% 195.4 / 11.8 95V43G (GTT→GGT) Clo1313_1378stage III sporulation protein AC
Reads supporting (aligned to +/- strand):  ref base T (19/50);  new base G (26/0);  total (45/50)
Fisher's exact test for biased strand distribution p-value = 1.63e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

Only 100 of 102 total aligned reads displayed.

ACACGGAGGAGAAAAAGAAGTATGAATATAGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCGGATTGGCTTTGGTTGCTACAGTAATAGCTTTGCT  >  CP002416/1620149‑1620445
                                                                                                                                                      |                                                                                                                                                     
acacGGAGGAGAAAAAGAAGTATGAATATAGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑tt                                                                                                                                                      <  1:2357115/150‑1 (MQ=255)
  acGGAGGAGAAAAAGAAGTATGAATATAGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑tttt                                                                                                                                                    <  1:872275/150‑1 (MQ=255)
  acGGAGGAGAAAAAGAAGTATGAATATAGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑tttt                                                                                                                                                    <  1:528798/150‑1 (MQ=255)
    ggaggaGAAAAAGAAGTATGAATATAGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTtga                                                                                                                                                  <  1:1507579/150‑1 (MQ=255)
         agaAAAAGAAGTATGAATATAGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTtgatgatg                                                                                                                                             <  1:2833611/150‑1 (MQ=255)
          gaAAAAGAAGTATGAATATAGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTtgatgatga                                                                                                                                            <  1:528367/150‑1 (MQ=255)
           aaaaaGAAGTATGAATATAGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTgatgatgat                                                                                                                                           <  1:410920/150‑1 (MQ=255)
             aaaGAAGTATGAATATAGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑gttt                                                                                                                                                    >  1:1811610/1‑139 (MQ=255)
             aaaGAAGTATGAATATAGAGTTGATTTTCAGAATTGCGGCAATAGGAAGTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAg                                                                                                                                         <  1:401010/150‑1 (MQ=255)
              aagaagTATGAATATAGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGGTTGG‑GTGTGG‑gttt                                                                                                                                                    >  1:1900168/1‑138 (MQ=255)
              aagaagTATGAATATAGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑gttt                                                                                                                                                    >  1:823676/1‑138 (MQ=255)
                     aTGAATATAGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGaa                                                                                                                                 <  1:2067871/150‑1 (MQ=255)
                     aTGAATATAGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑GTGTGG‑gttt                                                                                                                                                    >  1:838915/1‑131 (MQ=255)
                      tGAATATAGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑GTTTGATGGTGGTAggaaaggaaa                                                                                                                                >  1:1007695/1‑150 (MQ=255)
                         atatAGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAg                                                                                                                             <  1:1158658/150‑1 (MQ=255)
                           ataGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCa                                                                                                                           <  1:518849/150‑1 (MQ=255)
                           ataGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑GTGTGG‑GTTTGATGAAGATAgga                                                                                                                                       >  1:317283/1‑136 (MQ=255)
                            taGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCaa                                                                                                                          <  1:1306688/150‑1 (MQ=255)
                            taGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCaa                                                                                                                          <  1:2494437/150‑1 (MQ=255)
                             aGATTTGATTTTCAGAATTGCGTCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACAGGCGGGATTGG‑TTGTGGGTTTTG‑TGATgaaaggaaagtcaaaagcaaa                                                                                                                         >  1:783703/1‑139 (MQ=255)
                             aGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGGTTTG‑GTGTGG‑GTTTGATGATGGTAGTAAAGGAAATAGCaaa                                                                                                                         >  1:1760640/1‑150 (MQ=255)
                             aGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCaaa                                                                                                                         <  1:286632/150‑1 (MQ=255)
                              gatttgatttTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGGTTGG‑TTGGGG‑GTTTGATGATGATAGTAAAGGAAATAACAAAc                                                                                                                        >  1:1638350/1‑150 (MQ=255)
                              gatttgatttTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAAc                                                                                                                        <  1:2157856/150‑1 (MQ=255)
                                tttgatttTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCt                                                                                                                      <  1:693225/150‑1 (MQ=255)
                                    atttTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGttt                                                                                                                  <  1:2232984/150‑1 (MQ=255)
                                    atttTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGttt                                                                                                                  <  1:1537332/150‑1 (MQ=255)
                                    atttTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGttt                                                                                                                  <  1:267377/150‑1 (MQ=255)
                                    atttTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGttt                                                                                                                  <  1:2657007/150‑1 (MQ=255)
                                     ttttCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTg                                                                                                                 <  1:2171372/150‑1 (MQ=255)
                                      tttCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGa                                                                                                                <  1:487912/150‑1 (MQ=255)
                                      tttCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGa                                                                                                                <  1:1567799/150‑1 (MQ=255)
                                         cAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGGTTGG‑GTGTGG‑GTTTGATGATGATAGGAAAAGAAATAGCAAAACTGtt                                                                                                                   >  1:2892777/1‑144 (MQ=255)
                                          aGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGcc                                                                                                            <  1:1791647/150‑1 (MQ=255)
                                            aaTTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGGTTGG‑GTGTGG‑TTTTGTTGATGATAGTAAAGGAAATAGAAAAcc                                                                                                                       >  1:1152077/1‑137 (MQ=255)
                                               tGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑Taaa                                                                                                      <  1:2137912/150‑1 (MQ=255)
                                                gCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑Taaaa                                                                                                     <  1:1299059/150‑1 (MQ=255)
                                                 cGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGAGTGG‑GTGTGG‑GTTTTATGAGGATAGTAAAgg                                                                                                                                   >  1:2479706/1‑120 (MQ=255)
                                                   gCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGGGGGTTTTG‑TGATGATAGTAAAGGAAATAGCAAACCCGTTTGATGGCG‑Taaaaa                                                                                                    >  1:2160475/1‑148 (MQ=255)
                                                      aTAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTg                                                                                               <  1:3169350/150‑1 (MQ=255)
                                                        aGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGtt                                                                                             <  1:80832/150‑1 (MQ=255)
                                                        aGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑GTTTGATGATGATAGTAAAGGGAAAAGCAAAACTGTTTGATGCCG‑Taaaaa                                                                                                    >  1:2055820/1‑143 (MQ=255)
                                                         ggAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGGTTGG‑GTGTGG‑GTTTGTTGATGATAGGAAAGGAAATAACAAACCTGTTTTATGGCG‑Ttaaaa                                                                                                    >  1:105385/1‑142 (MQ=255)
                                                          gAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGGTGGG‑GTGTGG‑GTTTGGTGATGGTAggaaaggaaa                                                                                                                                >  1:636131/1‑114 (MQ=255)
                                                          gAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑GTTTGATGGTGCTAGTAAAGGAAATAACAAACCTtttt                                                                                                                  >  1:3056746/1‑128 (MQ=255)
                                                           aaTTTTGGTCTCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGGGTGG‑TTGGGG‑GTTTGATGATGATAGTAAAGGAAATAGCAAAACTGGTTGATGCCG‑GAAAAacaatg                                                                                               >  1:1820815/1‑142 (MQ=255)
                                                            aTTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAg                                                                                         <  1:1668092/150‑1 (MQ=255)
                                                                tGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGtttt                                                                                     <  1:1705371/150‑1 (MQ=255)
                                                                tGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑GTGTGG‑TTTTGATGATGATAGGAAAGGGAAAAGAAAAACTGTTTTATGACG‑TAAAAAAACTGTTTTAGtttt                                                                                     >  1:857614/1‑150 (MQ=255)
                                                                   tATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGGTTGG‑GTGTGG‑GTTTGATGATGATAGTAAAGGGAATAGCAAACCTGTTTGGTGGCG‑TAAAAACACCGGTTCAGTTTTAAg                                                                                  >  1:2322904/1‑150 (MQ=255)
                                                                    aTCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑GTGTGGGTTTTG‑TGATGAAAGTAAAGGGAAAAGTAAAACCGTTTTATGCCGGTAAAAA‑ACTGTTTCAGTTTTAAGa                                                                                 >  1:1799030/1‑150 (MQ=255)
                                                                       tGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGGTTGGGTGGGGG‑TTTTG‑TGATGATAGGAAAGGAAAAAGCAAAACTGTTTGTTGCCG‑TAAAAACACTGGTTTAGATTTAAGAttt                                                                              >  1:2523014/1‑150 (MQ=255)
                                                                       tGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGAttt                                                                              <  1:3167811/150‑1 (MQ=255)
                                                                        gTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTtc                                                                             <  1:93219/150‑1 (MQ=255)
                                                                          tttGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑GTGGGG‑TTTTGGTGATGATAGGAAAGGAAATAGGAAAACTGTTTTATGCCG‑GAAAAACACTGTTTCAGGTTTAAGAttt                                                                              >  1:1972689/1‑147 (MQ=255)
                                                                              aaTGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑GTGTGG‑TTTTGATGATGATAGGAAAAGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTTTCagag                                                                       >  1:712842/1‑150 (MQ=255)
                                                                              aaTGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑GTGTGG‑GTTTGATGGTGATAGGAAAGGAAAAAGCAAACCTGTTTTATGCCG‑TAAAAACACTGTTTTAGTTTTAAGATTTTTCagag                                                                       >  1:1905120/1‑150 (MQ=255)
                                                                               aTGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAgg                                                                      <  1:1631812/150‑1 (MQ=255)
                                                                                 gCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑GTGGGG‑GTTTGGTGATGGTAGGAAAGGAAAAAGGAAACCTGTTTGGTGGCG‑TAAAAACACTGTTTCAGGTTTAAGAATTTTCAGAgg                                                                      >  1:425111/1‑148 (MQ=255)
                                                                                   aTTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGga                                                                  <  1:2849750/150‑1 (MQ=255)
                                                                                    ttGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑GTTTGATGGTGATAGTAAAGGAAAAAGCAAACCTGTTTGATGCCG‑TAAAAAAACTGTTTCAGGTTTAAGATTTCTCag                                                                         >  1:1155845/1‑142 (MQ=255)
                                                                                    ttGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑GTGTGG‑GTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑GAAAAACACTGTTTCAGGTTTAAGATTTCTCaga                                                                        >  1:3022104/1‑143 (MQ=255)
                                                                                      gCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGgatga                                                               <  1:1708580/150‑1 (MQ=255)
                                                                                       cTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGGTTGG‑GTGTGG‑GTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAAAACTGTTTCAGTTTTAAGATTTCTCAGAggggg                                                                   >  1:190086/1‑142 (MQ=255)
                                                                                         gAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑GTTTGATGATGATAGTAAAGGAAATAGCAAAACTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGAATTCTCag                                                                         >  1:2160642/1‑137 (MQ=255)
                                                                                          aaCAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGGGTGG‑TTGTGG‑GTTTGTTGGTGATTGTAAAGGAAATATTAAAACCGTTTGACGCCG‑CAAAAACACCGGTTCAGGTTTAAGATTtctccga                                                                        >  1:1350656/1‑134 (MQ=255)
                                                                                          aaCAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATaaa                                                           <  1:1941826/150‑1 (MQ=255)
                                                                                           aCAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑GTGTGG‑TTTTGATGATGATATAAAAGGGAATAGCAAAACTGTTTGATGCCG‑Taaaaa                                                                                                    >  1:2343349/1‑108 (MQ=255)
                                                                                              aaTCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATg                                                       <  1:1507577/150‑1 (MQ=255)
                                                                                              aaTCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATg                                                       <  1:1754139/150‑1 (MQ=255)
                                                                                                   ggaagggaagAACAGGCAATGATGACCACACTGGCGGGATTTG‑TTGTGG‑TTTTTTTGATGATAGTAAAAGAAATAGCAAACCTGTTTTATGCCG‑TAAAAACACTGTTTTAGTTTTAAGATTTCTCAGAgg                                                                      >  1:3080556/1‑130 (MQ=255)
                                                                                                    gaagggaagAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAata                                                 <  1:1784918/150‑1 (MQ=255)
                                                                                                     aagggaagAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAatat                                                <  1:2160934/150‑1 (MQ=255)
                                                                                                      agggaagAACAGGCAATGATGACCACACTGGCGGGGTTGG‑TTGTGG‑GTTTGATGATGATAGTAAAGGAAAAAGCAAACCTGTTTTATGCCG‑GAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGGGGATGATAAAAAAGGAAAAAtt                                               >  1:409370/1‑150 (MQ=255)
                                                                                                       gggaagAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATAttt                                              <  1:1238666/150‑1 (MQ=255)
                                                                                                         gaagaaCAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCa                                            <  1:2055841/150‑1 (MQ=255)
                                                                                                            gaaCAGGCAATGATGACCACACTGGCGGGATTGG‑GTGTGG‑GTTTGGTGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAAAGGAAATATTTTAGAt                                         >  1:1413121/1‑150 (MQ=255)
                                                                                                                 ggCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCg                                    <  1:901840/150‑1 (MQ=255)
                                                                                                                   cAATGATGACCACACTGGCGGGGTTGG‑GTGTGG‑GTTTGATGGTGATAGGAAAGGAAAAAGCAAAACTGTTTGATGCCG‑GAAAAACACTGTTTTCGTTTTTAGATTTCTCAGAGGCGGGTGGTaaaaaagggaaaattt                                              >  1:1524428/1‑127 (MQ=255)
                                                                                                                     atgatgACCACACTGGCGGGGTTGG‑TTGTGG‑GTTTGGTGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGGTGATAAAAAAGGAAATATTTCAGATTATCgg                                   >  1:1120893/1‑147 (MQ=255)
                                                                                                                      tgatgaCCACACTGGCGGGATTGG‑GTGGGG‑GTTTTATGATGATAGTAAAAGAAAAAACAAAACCGTTTGGTGGCG‑GAAAAACAATGTTTTAGATTTAAGAATTCTCAGAGGCGGGTGATaaaaa                                                         >  1:2416216/1‑124 (MQ=255)
                                                                                                                       gatgaCCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCGGATTgg                              <  1:773362/150‑1 (MQ=255)
                                                                                                                         tgaCCACACTGGCGGGATTCG‑GTGTTG‑GTTTGATGATGCTAGTAAAGGAAATAGCCAAACTGTTTTATGCCA‑TAAAAACACCGTTTCCGTTTTTAGATTTTTCTGAggggggt                                                                 >  1:380930/1‑108 (MQ=255)
                                                                                                                          gaCCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCGGATTGGCtt                           <  1:233508/150‑1 (MQ=255)
                                                                                                                             cacaCTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCGGATTGGCTTTgg                        <  1:1806541/150‑1 (MQ=255)
                                                                                                                                   ggCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCGGATTGGCTTTGGTTGCTa                  >  1:1957246/1‑150 (MQ=255)
                                                                                                                                   ggCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCGGATTGGCTTTGGTTGCTa                  <  1:2022636/150‑1 (MQ=255)
                                                                                                                                     cGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCGGATTGGCTTTGGTTGCTACa                <  1:1218169/150‑1 (MQ=255)
                                                                                                                                       ggATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCGGATTGGCTTTGGTTGCTACAGt              <  1:2418271/150‑1 (MQ=255)
                                                                                                                                       ggATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCGGATTGGCTTTGGTTGCTACAGt              >  1:269770/1‑150 (MQ=255)
                                                                                                                                        gATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCGGATTGGCTTTGGTTGCTACAGTa             <  1:1114939/150‑1 (MQ=255)
                                                                                                                                        gATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCGGATTGGCTTTGGTTGCTACAGTa             >  1:2457831/1‑150 (MQ=255)
                                                                                                                                            gg‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCATATTATCGGATTGGCTTTGGTTGCTACAGTAATa          <  1:441163/149‑1 (MQ=255)
                                                                                                                                               ttGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCGGATTGGCTTTGGTTGCTACAGTAATAGCt       >  1:1538518/1‑150 (MQ=255)
                                                                                                                                                tgtgG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCGGATTGGCTTTGGTTGCTACAGTAATAGCtt      <  1:1467722/150‑1 (MQ=255)
                                                                                                                                                 gtgG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCGGATTGGCTTTGGTTGCTACAGTAATAGCttt     >  1:2302830/1‑150 (MQ=255)
                                                                                                                                                 gtgG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCGGATTGGCTTTGGTTGCTACAGTAATAGCttt     >  1:1062659/1‑150 (MQ=255)
                                                                                                                                                  tgG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCGGATTGGCTTTGGTTGCTACAGTAATAGCTTTg    >  1:3010280/1‑150 (MQ=255)
                                                                                                                                                   gg‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCGGATTGGCTTTGGTTGCTACAGTAATAGCTTTGc   >  1:2355747/1‑150 (MQ=255)
                                                                                                                                                    g‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTCAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCGGATTGGCTTTGGTTGCTACAGTAATAGCTTTGct  >  1:879708/1‑150 (MQ=255)
                                                                                                                                                      |                                                                                                                                                     
ACACGGAGGAGAAAAAGAAGTATGAATATAGATTTGATTTTCAGAATTGCGGCAATAGGAATTTTGGTATCTGTTTTGAATGCATTGCTGAACAAATCCGGAAGGGAAGAACAGGCAATGATGACCACACTGGCGGGATTGG‑TTGTGG‑TTTTGATGATGATAGTAAAGGAAATAGCAAACCTGTTTGATGCCG‑TAAAAACACTGTTTCAGTTTTAAGATTTCTCAGAGGCGGATGATAAAAATGGAAATATTTCAGATTATCGGATTGGCTTTGGTTGCTACAGTAATAGCTTTGCT  >  CP002416/1620149‑1620445

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑