Predicted mutation
evidence seq id position mutation annotation gene description
RA CP002416 2,838,567 G→A A73A (GCC→GCT)  Clo1313_2428 ← periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0024162,838,5670GA86.1% 494.5 / 53.2 173A73A (GCC→GCT) Clo1313_2428periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base G (11/13);  new base A (76/72);  total (87/86)
Fisher's exact test for biased strand distribution p-value = 6.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 175 total aligned reads displayed.

TGAAGTTCAACTATCTTTTCTACATTCGGCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGGGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCGTTTTTATTGTTTTTATTGCATGAAGAAAAAGCAA  >  CP002416/2838418‑2838715
                                                                                                                                                     |                                                                                                                                                    
tGAAGTTCAACTATCTTTTCTACATTCGGCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTggggg                                                                                                                                                     <  1:2034550/151‑1 (MQ=255)
  aaGTTCAACTATCTTTTCTACATTCGGCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGGGCa                                                                                                                                                   <  1:4044268/151‑1 (MQ=255)
    gTTCAACTATCTTTTCTACATTCGGCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGGGCAAc                                                                                                                                                 >  1:3238032/1‑151 (MQ=255)
     ttCAACTATCTTTTCTACATTCGGCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACt                                                                                                                                                <  1:863508/151‑1 (MQ=255)
       cAACTATCTTTTCTACATTCGGCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTga                                                                                                                                              <  1:2008233/151‑1 (MQ=255)
        aaCTATCTTTTCTACATTCGGCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTgag                                                                                                                                             <  1:3909514/151‑1 (MQ=255)
         aCTATCTTTTCTACATTCGGCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTgaga                                                                                                                                            >  1:3893094/1‑151 (MQ=255)
          cTATCTTTTCTACATTCGGCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGTCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGAc                                                                                                                                           <  1:4012925/151‑1 (MQ=255)
             tCTTTTCTACATTCGGCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGGGCAACTGAGACTAt                                                                                                                                        >  1:1455114/1‑151 (MQ=255)
               ttttCTACATTCGGCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGGGCAACTGAGACTATcc                                                                                                                                      >  1:4297243/1‑151 (MQ=255)
               ttttCTACATTCGGCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATcc                                                                                                                                      <  1:1885899/151‑1 (MQ=255)
                 ttCTACATTCGGCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCtt                                                                                                                                    >  1:1094181/1‑151 (MQ=255)
                    tACATTCGGCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCa                                                                                                                                 >  1:3596325/1‑151 (MQ=255)
                     aCATTCGGCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGGGCAACTGAGACTATCCTTTCAg                                                                                                                                >  1:2176509/1‑151 (MQ=255)
                      cATTCGGCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAgg                                                                                                                               >  1:271057/1‑151 (MQ=255)
                         tCGGCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTc                                                                                                                            <  1:2184619/151‑1 (MQ=255)
                          cGGCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGGGCAACTGAGACTATCCTTTCAGGTTCt                                                                                                                           >  1:437626/1‑151 (MQ=255)
                           ggCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCtt                                                                                                                          >  1:1567072/1‑151 (MQ=255)
                             cTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTg                                                                                                                        >  1:1653135/1‑151 (MQ=255)
                              tGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGt                                                                                                                       >  1:2255916/1‑151 (MQ=255)
                               ggTCTATGTTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTc                                                                                                                      >  1:501580/1‑151 (MQ=255)
                                 tCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCa                                                                                                                    >  1:1063430/1‑151 (MQ=255)
                                    atatTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGt                                                                                                                 >  1:1719233/1‑151 (MQ=255)
                                     tatTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGtt                                                                                                                <  1:3976717/151‑1 (MQ=255)
                                      atTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTg                                                                                                               >  1:1519504/1‑151 (MQ=255)
                                           cTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTc                                                                                                          >  1:1899361/1‑151 (MQ=255)
                                             aTTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCAGTCCGTCCCACACGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGTGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACttaac                                                                                                        >  1:3560440/1‑148 (MQ=255)
                                              ttGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCt                                                                                                       >  1:3473471/1‑151 (MQ=255)
                                                  cTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCa                                                                                                   <  1:68277/151‑1 (MQ=255)
                                                     gATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAg                                                                                                >  1:1009812/1‑151 (MQ=255)
                                                     gATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAg                                                                                                <  1:3368174/151‑1 (MQ=255)
                                                     gATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAg                                                                                                <  1:793544/151‑1 (MQ=255)
                                                         ttcttcGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGGGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAAt                                                                                            <  1:3790294/151‑1 (MQ=255)
                                                           cttcGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATcc                                                                                          <  1:185243/151‑1 (MQ=255)
                                                            ttcGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCt                                                                                         <  1:2340660/151‑1 (MQ=255)
                                                            ttcGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCt                                                                                         >  1:3099312/1‑151 (MQ=255)
                                                              cGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTc                                                                                       >  1:1439756/1‑151 (MQ=255)
                                                               gCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCa                                                                                      >  1:133588/1‑151 (MQ=255)
                                                               gCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCa                                                                                      <  1:2460939/151‑1 (MQ=255)
                                                                 ctctTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCaaa                                                                                    >  1:3081439/1‑151 (MQ=255)
                                                                 ctctTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCaaa                                                                                    >  1:1948636/1‑151 (MQ=255)
                                                                  tctTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAg                                                                                   <  1:2658303/151‑1 (MQ=255)
                                                                  tctTCCGAATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGGGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAg                                                                                   >  1:462839/1‑151 (MQ=255)
                                                                   ctTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGt                                                                                  >  1:3465666/1‑151 (MQ=255)
                                                                        ggATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTacac                                                                             <  1:1007801/151‑1 (MQ=255)
                                                                         gATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACt                                                                            <  1:2215596/151‑1 (MQ=255)
                                                                            aaTCTCAAAAATCCGTCCGAGTGACCAGATTGTCATGCTTGCCCACCGCAAAAATTATCTCCGTTATGTTGGGGGCAACTGAGACTATCCTTTGAGGTTCTTTGCCCAGGTTGACTTCCCTGTAATAGGAAGCCTTCGAAGTTAAACTGTa                                                                         <  1:2795045/151‑1 (MQ=255)
                                                                               cGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGGGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATcc                                                                      <  1:1271750/151‑1 (MQ=255)
                                                                                     aaTCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGttatt                                                                <  1:291088/151‑1 (MQ=255)
                                                                                           tccgtccCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATc                                                          >  1:2949747/1‑151 (MQ=255)
                                                                                              gtccCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCAcc                                                       <  1:33237/151‑1 (MQ=255)
                                                                                              gtccCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCAcc                                                       >  1:3171777/1‑151 (MQ=255)
                                                                                               tccCTCCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCg                                                      <  1:1604996/151‑1 (MQ=255)
                                                                                                 ccaccaGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGc                                                    <  1:2762835/151‑1 (MQ=255)
                                                                                                 ccaccaGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGc                                                    >  1:2499724/1‑151 (MQ=255)
                                                                                                  caccaGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGcc                                                   >  1:2585047/1‑151 (MQ=255)
                                                                                                  caccaGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGcc                                                   >  1:3378492/1‑151 (MQ=255)
                                                                                                   accaGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGccc                                                  >  1:2022734/1‑151 (MQ=255)
                                                                                                    ccaGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGGGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGcccc                                                 <  1:650972/151‑1 (MQ=255)
                                                                                                      aGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGGGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCAt                                               >  1:1966226/1‑151 (MQ=255)
                                                                                                      aGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGGGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCAt                                               <  1:2467646/151‑1 (MQ=255)
                                                                                                          tGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGt                                           <  1:705814/151‑1 (MQ=255)
                                                                                                           gTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGtt                                          >  1:1079066/1‑151 (MQ=255)
                                                                                                           gTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGtt                                          <  1:3442028/151‑1 (MQ=255)
                                                                                                           gTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGtt                                          >  1:4183581/1‑151 (MQ=255)
                                                                                                             ccTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTgg                                        >  1:1086651/1‑151 (MQ=255)
                                                                                                                   tGCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCgtt                                  <  1:3951774/151‑1 (MQ=255)
                                                                                                                    gCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCgttt                                 >  1:1023086/1‑151 (MQ=255)
                                                                                                                    gCCCAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCgttt                                 <  1:3592114/151‑1 (MQ=255)
                                                                                                                      ccAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCgttttt                               <  1:426690/151‑1 (MQ=255)
                                                                                                                       cAACGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCgttttta                              >  1:1147146/1‑151 (MQ=255)
                                                                                                                         aCGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCgtttttatt                            <  1:805130/151‑1 (MQ=255)
                                                                                                                          cGCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCgtttttattg                           >  1:2362372/1‑151 (MQ=255)
                                                                                                                           gCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCgtttttattgt                          <  1:2739432/151‑1 (MQ=255)
                                                                                                                           gCAAAAATTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCgtttttattgt                          >  1:3136752/1‑151 (MQ=255)
                                                                                                                           gCAAAAATTATCTCCGTTATGTTGGGAGCAACAGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCgtttttattgt                          >  1:4161337/1‑151 (MQ=255)
                                                                                                                            cAAAAATTATCTCCGTTATGTTGGGGGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCgtatttaatgtt                         >  1:245000/1‑151 (MQ=255)
                                                                                                                             aaaaaTTATCTCCGTTATGTTGGGGGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCgtttttatagttt                        <  1:4201377/151‑1 (MQ=255)
                                                                                                                             aaaaaTTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCgtttttattgttt                        <  1:2099813/151‑1 (MQ=255)
                                                                                                                             aaaaaTTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCgtttttattgttt                        >  1:4141909/1‑151 (MQ=255)
                                                                                                                               aaaTTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCgtttttattgttttt                      <  1:6627/151‑1 (MQ=255)
                                                                                                                                aaTTATCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCgtttttattgttttta                     >  1:140346/1‑151 (MQ=255)
                                                                                                                                    aTCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCGTTTTTATTGTTTTTATTGc                 <  1:4235372/151‑1 (MQ=255)
                                                                                                                                    aTCTCCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCGTTTTTATTGTTCTTATTGc                 <  1:563793/151‑1 (MQ=255)
                                                                                                                                     tctcCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCGTTTTTATTGTTTTTATTGCa                <  1:3340296/151‑1 (MQ=255)
                                                                                                                                      ctcCGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCGTTTTTATTGTTTTTATTGCAt               <  1:71434/151‑1 (MQ=255)
                                                                                                                                        ccGTTATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCGTTTTTATTGTTTTTATTGCATga             <  1:287247/151‑1 (MQ=255)
                                                                                                                                           ttATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCGTTTTTATTGTTTTTATTGCATgaaga          <  1:3528772/151‑1 (MQ=255)
                                                                                                                                           ttATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCGTTTTTATTGTTTTTATTGCATgaaga          <  1:462040/151‑1 (MQ=255)
                                                                                                                                            tATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCGTTTTTATTGTTTTTATTGCATgaagaa         >  1:4105770/1‑151 (MQ=255)
                                                                                                                                            tATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCGTTTTTATTGTTTTTATTGCATgaagaa         >  1:366560/1‑151 (MQ=255)
                                                                                                                                            tATGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCGTTTTTATTGTTTTTATTGCATgaagaa         >  1:3384343/1‑151 (MQ=255)
                                                                                                                                             aTGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCGTTTTTATTGTTTTTATTGCATGAAGaaa        >  1:2412785/1‑151 (MQ=255)
                                                                                                                                             aTGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCGTTTTTATTGTTTTTATTGCATGAAGaaa        <  1:1598027/151‑1 (MQ=255)
                                                                                                                                              tGTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCGTTTTTATTGTTTTTATTGCATGAAGaaaa       <  1:316317/151‑1 (MQ=255)
                                                                                                                                               gTTGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCGTTTTTATTGTTTTTATTGCATGAAGaaaaa      <  1:2054835/151‑1 (MQ=255)
                                                                                                                                                ttGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCGTTTTTATTGTTTTTATTGCATGAAGAAAAAg     <  1:29093/151‑1 (MQ=255)
                                                                                                                                                ttGGGAGCAACTGAGACTATCCTTTCAGGTTCTTTATCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCGTTTTTATTGTTTTTATTGCATGAAGAAAAAg     <  1:4056004/151‑1 (MQ=255)
                                                                                                                                                 tGGGGGCAGCTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCTCCGGCCCCATCAGTTGGCCCGTTTTTATTGTTTTTATTGCATGAAGAAAAAGc    <  1:320321/151‑1 (MQ=255)
                                                                                                                                                   ggggCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCGTTTTTATTGTTTTTATTGCATGAAGAAAAAGCaa  <  1:1366214/151‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
TGAAGTTCAACTATCTTTTCTACATTCGGCTGGTCTATATTTCCTATTGACTCGATGTTCTTCGCCTCTTCCGGATAATCGCAAAAATCCGTCCGTCCCACCAGCTTGTCCTGCTTGCCCAACGCAAAAATTATCTCCGTTATGTTGGGGGCAACTGAGACTATCCTTTCAGGTTCTTTGTCCAGGTTGACTTCCCTGTCATAGGAATCCTTCAAAGTTACACTGTATCCGTTATTATTATCACCGGCCCCATCAGTTGGCCCGTTTTTATTGTTTTTATTGCATGAAGAAAAAGCAA  >  CP002416/2838418‑2838715

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑