BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP002416	2,704,920	(T)_7→8	intergenic (‑1451/+836)	Clo1313_2294 ← / ← Clo1313_2296	two component transcriptional regulator, winged helix family/flavin reductase domain protein FMN‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP002416	2,704,920	1	.	T	98.3%	228.9 / ‑0.8	120	intergenic (‑1451/+836)	Clo1313_2294/Clo1313_2296	two component transcriptional regulator, winged helix family/flavin reductase domain protein FMN‑binding protein
Reads supporting (aligned to +/- strand): ref base . (0/2); new base T (64/54); total (64/56)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.79e-01

Only 100 of 127 total aligned reads displayed.

TCATAATCCCTTGAGTCAATCATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTT‑AAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAATTTATTGACAGTCGTACAATTTCATCCTCAAGACGACGGGTTTTCTTCGCTTTATAGGAGAT  >  CP002416/2704771‑2705060

acATAATCCCTTGAGTCAATCATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCtttttttt                                                                                                                                              >  1:3253037/2‑150 (MQ=21)

 cATAATCCCTTGAGTCAATCATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCtttttttta                                                                                                                                             <  1:699696/151‑3 (MQ=18)

   tAATCCCTTGAGTCAATCATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCttttttttaag                                                                                                                                           <  1:2370879/151‑5 (MQ=17)

   tAATCCCTTGAGTCAATCATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCttttttttaag                                                                                                                                           >  1:4294518/1‑147 (MQ=17)

   tAATCCCTTGAGTCAATCATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCttttttttaag                                                                                                                                           <  1:3884337/151‑5 (MQ=17)

    aaTCCCTTGAGTCAATCATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCttttttttaaga                                                                                                                                          <  1:920400/151‑6 (MQ=14)

        ccTTGAGTCAATCATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACATCCGGTGTTTCTTTTTTTTAAGAGAtt                                                                                                                                      <  1:3977866/151‑1 (MQ=9)

          ttGAGTCAATCATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGc                                                                                                                                    >  1:3644286/1‑151 (MQ=14)

          ttGAGTCAATCATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGc                                                                                                                                    >  1:1507664/1‑151 (MQ=14)

            gAGTCAATCATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCaa                                                                                                                                  >  1:3750512/1‑151 (MQ=14)

            gAGTCAATCATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCaa                                                                                                                                  <  1:3838077/151‑1 (MQ=14)

             aGTCAATCATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCaaa                                                                                                                                 >  1:864002/1‑151 (MQ=14)

                cAATCATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTg                                                                                                                              <  1:269832/151‑1 (MQ=14)

                cAATCATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTg                                                                                                                              >  1:3925958/1‑151 (MQ=14)

                 aaTCATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGt                                                                                                                             >  1:4199109/1‑151 (MQ=14)

                  aTCATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTa                                                                                                                            <  1:2185510/151‑1 (MQ=14)

                   tCATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTAc                                                                                                                           >  1:432134/1‑151 (MQ=14)

                    cATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACt                                                                                                                          <  1:3644022/151‑1 (MQ=14)

                      tatCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACttt                                                                                                                        <  1:3532707/151‑1 (MQ=14)

                       atCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACtttt                                                                                                                       >  1:1690145/1‑151 (MQ=14)

                        tCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTa                                                                                                                      <  1:1442423/151‑1 (MQ=14)

                        tCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTa                                                                                                                      <  1:1163939/151‑1 (MQ=14)

                          aaGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTAcc                                                                                                                    <  1:4222724/151‑1 (MQ=14)

                            gTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGa                                                                                                                  >  1:1765405/1‑151 (MQ=14)

                              aTTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGata                                                                                                                >  1:2590758/1‑151 (MQ=14)

                               tttGATGCGTCAAAATATCAATCATAATTGTTCCATAGCTTTTTCTCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGCTACTGTCTTTTTGACAACAACCGGTGATTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACTGatat                                                                                                               >  1:1838022/1‑151 (MQ=11)

                               tttGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGatat                                                                                                               <  1:972734/151‑1 (MQ=14)

                                 tGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATcc                                                                                                             >  1:680209/1‑151 (MQ=14)

                                 tGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATcc                                                                                                             >  1:3054190/1‑151 (MQ=14)

                                  gATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCca                                                                                                            <  1:2071236/151‑1 (MQ=14)

                                     gCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCacaa                                                                                                         <  1:1644082/151‑1 (MQ=14)

                                      cGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCacaac                                                                                                        >  1:3672013/1‑151 (MQ=14)

                                       gTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCacaaca                                                                                                       >  1:2130501/1‑151 (MQ=14)

                                         aaaaaTATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACAtt                                                                                                     >  1:602560/1‑151 (MQ=14)

                                         aaaaaTATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACAtt                                                                                                     <  1:1851080/151‑1 (MQ=14)

                                            aaTATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTc                                                                                                  <  1:3065987/151‑1 (MQ=14)

                                            aaTATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTc                                                                                                  >  1:2615236/1‑151 (MQ=14)

                                             atatCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAATCAGCTGTTACTGTCTTTTTGTCAACATCCGGTGTTTCTTTTTTTTAAGAGATTGCAAGCTGTACTTTTACCGATATCCACAACATTTTcc                                                                                                 <  1:4098068/151‑1 (MQ=11)

                                             atatCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTcc                                                                                                 <  1:692884/151‑1 (MQ=14)

                                             atatCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTcc                                                                                                 >  1:1763484/1‑151 (MQ=14)

                                             atatCTATCATAATTGCTCCATAGCTTTTTCGCTTTTTAATAGCATAATCATCAATGCAAACAGTTGTTACTGTTTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGTTTGCAAACTGTACTTTTACCGTTATCCACAACAtttttc                                                                                                 >  1:2369061/1‑149 (MQ=11)

                                              tatctatcATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTctt                                                                                                <  1:3770042/151‑3 (MQ=9)

                                              tatctatcATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCt                                                                                                <  1:1303821/151‑1 (MQ=14)

                                               atctatcATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCtt                                                                                               >  1:3343254/1‑151 (MQ=14)

                                               atctatcATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCtt                                                                                               >  1:1850325/1‑151 (MQ=14)

                                               atctatcATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCtt                                                                                               >  1:1352541/1‑151 (MQ=14)

                                                tctatcATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCttt                                                                                              <  1:3391134/151‑1 (MQ=14)

                                                 ctatcATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTa                                                                                             <  1:1684969/151‑1 (MQ=14)

                                                 ctatcATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTa                                                                                             <  1:4246491/151‑1 (MQ=14)

                                                 ctatcATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTa                                                                                             >  1:1101647/1‑151 (MQ=14)

                                                 ctatcATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTa                                                                                             <  1:751692/151‑1 (MQ=14)

                                                  tatcATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGTCGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTag                                                                                            >  1:2398465/1‑151 (MQ=2)

                                                  tatcATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTag                                                                                            <  1:4195118/151‑1 (MQ=14)

                                                  tatcATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTag                                                                                            >  1:1493476/1‑151 (MQ=14)

                                                  tatcATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTag                                                                                            >  1:3231299/1‑151 (MQ=14)

                                                    tcATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTagag                                                                                          <  1:1832300/151‑1 (MQ=14)

                                                     cATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGc                                                                                         >  1:1558742/1‑151 (MQ=14)

                                                      aTAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCt                                                                                        >  1:655855/1‑151 (MQ=14)

                                                       tAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCtt                                                                                       >  1:1118103/1‑151 (MQ=14)

                                                         aTTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCtttt                                                                                     <  1:2722421/151‑1 (MQ=14)

                                                         aTTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCtttt                                                                                     >  1:3824770/1‑151 (MQ=14)

                                                         aTTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTCGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCtttt                                                                                     >  1:575021/1‑151 (MQ=9)

                                                          ttGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTg                                                                                    >  1:1867062/1‑151 (MQ=14)

                                                          ttGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTg                                                                                    <  1:457401/151‑1 (MQ=14)

                                                           tGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGa                                                                                   >  1:3698367/1‑151 (MQ=14)

                                                              tCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGAtgc                                                                                >  1:3268912/1‑151 (MQ=14)

                                                                cttAGCTTTTTTGTTGTTTAATAGCAAAATCATCAAAGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGCCTTTTCTTTTTTTTAAGAGATTGCAAATTGTACTTTTACAGATATCCACAACCTGTTCGTTTAGGGCTTTTGAtgctg                                                                              >  1:770854/3‑151 (MQ=2)

                                                                cgatcttttttCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGAtgctg                                                                              <  1:1667856/145‑1 (MQ=9)

                                                                 aTAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGAtgctgc                                                                             >  1:2965230/1‑151 (MQ=14)

                                                                   aGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCaa                                                                           >  1:697157/1‑151 (MQ=14)

                                                                     cTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACg                                                                         <  1:3127675/151‑1 (MQ=14)

                                                                        tttCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGaa                                                                      <  1:2780956/151‑1 (MQ=14)

                                                                        tttCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGaa                                                                      >  1:2632313/1‑151 (MQ=14)

                                                                        tttCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGaa                                                                      <  1:1729980/151‑1 (MQ=14)

                                                                           cGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTg                                                                   >  1:1752923/1‑151 (MQ=14)

                                                                             cTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCa                                                                 >  1:1158525/1‑151 (MQ=14)

                                                                              tttttAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCaa                                                                <  1:2516742/151‑1 (MQ=14)

                                                                               ttttAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAAt                                                               <  1:3394744/151‑1 (MQ=14)

                                                                               ttttAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAAt                                                               <  1:353216/151‑1 (MQ=14)

                                                                               ttttAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAAt                                                               <  1:2219614/151‑1 (MQ=14)

                                                                                tttAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAAtt                                                              <  1:2781512/151‑1 (MQ=14)

                                                                                tttAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAAtt                                                              <  1:3455543/151‑1 (MQ=14)

                                                                                   aaTAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAATTTAt                                                           <  1:1547523/151‑1 (MQ=14)

                                                                                     tAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAATTTATTg                                                         <  1:1440673/151‑1 (MQ=14)

                                                                                     tAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACAGAACTGCAATTTATTg                                                         <  1:2698964/151‑1 (MQ=9)

                                                                                       gCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAATTTATTGAc                                                       >  1:2817270/1‑151 (MQ=14)

                                                                                           aaTCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAATTTATTGACAGTc                                                   <  1:1364177/151‑1 (MQ=14)

                                                                                             tcatcaATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAATTTATTGACAGTCGt                                                 >  1:622252/1‑151 (MQ=14)

                                                                                               atcaATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAATTTATTGACAGTCGTAc                                               >  1:1920230/1‑151 (MQ=14)

                                                                                                 caATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAATTTATTGACAGTCGTACaa                                             >  1:4120362/1‑151 (MQ=14)

                                                                                                   aTGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAATTTATTGACAGTCGTACAAtt                                           <  1:1347797/151‑1 (MQ=14)

                                                                                                   aTGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAATTTATTGACAGTCGTACAAtt                                           <  1:4188488/151‑1 (MQ=14)

                                                                                                   aTGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAATTTATTGACAGTCGTACAAtt                                           >  1:3138903/1‑151 (MQ=14)

                                                                                                       aaaCAGCTGTTACTGTCTTTTTGTCAACACCCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAATTTATTGACAGTCGTACAATTTCAt                                       <  1:1926431/151‑1 (MQ=9)

                                                                                                       aaaCAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAATTTATTGACAGTCGTACAATTTCAt                                       >  1:502669/1‑151 (MQ=14)

                                                                                                       aaaCAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAATTTATTGACAGTCGTACAATTTCAt                                       >  1:3269492/1‑151 (MQ=14)

                                                                                                        aaCAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAATTTATTGACAGTCGTACAATTTCATc                                      >  1:2340355/1‑151 (MQ=14)

                                                                                                         aCAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAATTTATTGACAGTCGTACAATTTCATcc                                     <  1:2120950/151‑1 (MQ=14)

                                                                                                          cAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTTTAAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAATTTATTGACAGTCGTACAATTTCATCCt                                    >  1:2716847/1‑151 (MQ=11)

                                                                                                                                           ttccTTTTTTT‑AAGATATTGCAAACTGTACTTTTGCCGATATCCACAACATTTTCCTTTGTAGCTTGTGATTTTGCAACGGAACTGTAATTTATTGACAGTTGTACAATTTCATCCTCAAGTTGCCGTTTTTTCTTCGCTTTATAGGAGAt  <  1:312724/148‑1 (MQ=2)

TCATAATCCCTTGAGTCAATCATATCAAGTATTTGATGCGTAAAAATATCTATCATAATTGTTCCATAGCTTTTTCGCTTTTTAATAGCAAAATCATCAATGCAAACAGCTGTTACTGTCTTTTTGTCAACAACCGGTGTTTCTTTTTTT‑AAGAGATTGCAAACTGTACTTTTACCGATATCCACAACATTTTCCTTTAGAGCTTTTGATGCTGCAACGGAACTGCAATTTATTGACAGTCGTACAATTTCATCCTCAAGACGACGGGTTTTCTTCGCTTTATAGGAGAT  >  CP002416/2704771‑2705060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`