Predicted mutation
evidence seq id position mutation annotation gene description
RA CP002416 1,786,024 C→T R203H (CGT→CAT)  Clo1313_1529 ← agmatinase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0024161,786,0240CT99.2% 468.0 / NA 120R203H (CGT→CAT) Clo1313_1529agmatinase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (53/65);  total (55/65)
Fisher's exact test for biased strand distribution p-value = 4.54e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

CTTGAACAGATGTATTGAATCCAAAAGTTCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACACGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGTCAATCAAGCGTCTTACAGCCGAAGCA  >  CP002416/1785879‑1786170
                                                                                                                                                 |                                                                                                                                                  
cTTGAACAGATGTATTGAATCCAAAAGTTCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCtt                                                                                                                                               <  1:2292342/151‑1 (MQ=255)
cTTGAACAGATGTATTGAATCCAAAAGTTCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCtt                                                                                                                                               <  1:1792031/151‑1 (MQ=255)
  tGAACAGATGTATTGAATCCAAAAGTTCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTaa                                                                                                                                             <  1:1277589/151‑1 (MQ=255)
        gATGTATTGAATCCAAAAGTTCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTc                                                                                                                                       >  1:2474212/1‑151 (MQ=255)
         ttGTATTGAATCCAAAAGTTCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCa                                                                                                                                      <  1:2852231/150‑1 (MQ=255)
         aTGTATTGAATCCAAAAGTTCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCa                                                                                                                                      >  1:2091413/1‑151 (MQ=255)
         aTGTATTGAATCCAAAAGTTCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCa                                                                                                                                      >  1:822241/1‑151 (MQ=255)
          tGTATTGAATCCAAAAGTTCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCaa                                                                                                                                     >  1:956448/1‑151 (MQ=255)
          tGTATTGAATCCAAAAGTTCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACAGGGCTTAACGGCTCaa                                                                                                                                     >  1:1979987/1‑151 (MQ=255)
            tATTGAATCCAAAAGTTCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCaaaa                                                                                                                                   >  1:1560159/1‑151 (MQ=255)
            tATTGAATCAAAAAGTTCCCTGGCGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCaaaa                                                                                                                                   <  1:2874155/151‑1 (MQ=255)
             aTTGAATCCAAAATTTCCCGGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCaaaaa                                                                                                                                  <  1:493961/151‑1 (MQ=255)
               tGAATCCAAAAGTTCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACg                                                                                                                                <  1:1761281/151‑1 (MQ=255)
               tGAATCCAAAAGTTCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACg                                                                                                                                <  1:1420503/151‑1 (MQ=255)
                 aaTCCAAAAGTTCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTc                                                                                                                              >  1:1577353/1‑151 (MQ=255)
                  aTCCAAAAGTTCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCg                                                                                                                             <  1:666835/151‑1 (MQ=255)
                     cAAAAGTTCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATa                                                                                                                          <  1:3026529/151‑1 (MQ=255)
                       aaaGTTCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGt                                                                                                                        >  1:1065215/1‑151 (MQ=255)
                        aaGTTCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGta                                                                                                                       <  1:2234130/151‑1 (MQ=255)
                           ttCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATAc                                                                                                                    <  1:1941799/151‑1 (MQ=255)
                              ccTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATg                                                                                                                 <  1:1473112/151‑1 (MQ=255)
                               cTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGt                                                                                                                <  1:163606/151‑1 (MQ=255)
                                tGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGtt                                                                                                               <  1:2014179/151‑1 (MQ=255)
                                   aGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGt                                                                                                            >  1:1125814/1‑151 (MQ=255)
                                    gCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTa                                                                                                           <  1:808705/151‑1 (MQ=255)
                                     cTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTAt                                                                                                          >  1:2483269/1‑151 (MQ=255)
                                      tGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATg                                                                                                         >  1:2307018/1‑151 (MQ=255)
                                        aTCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGtt                                                                                                       <  1:1014189/151‑1 (MQ=255)
                                         tCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGttt                                                                                                      >  1:1901646/1‑151 (MQ=255)
                                           ccgccgGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGttttt                                                                                                    <  1:1443305/151‑1 (MQ=255)
                                           ccgccgGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGttttt                                                                                                    <  1:170782/151‑1 (MQ=255)
                                             gccgGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGttttttt                                                                                                  <  1:3187473/151‑1 (MQ=255)
                                               cgGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGc                                                                                                >  1:867783/1‑151 (MQ=255)
                                                tggCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCa                                                                                               >  1:2441807/2‑151 (MQ=255)
                                                gggCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCa                                                                                               >  1:492397/1‑151 (MQ=255)
                                                gggCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCa                                                                                               <  1:1901645/151‑1 (MQ=255)
                                                 ggCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCat                                                                                              <  1:1978203/151‑1 (MQ=255)
                                                   cTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCatat                                                                                            <  1:262559/151‑1 (MQ=255)
                                                         tgtACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAAc                                                                                      <  1:29576/151‑1 (MQ=255)
                                                           tACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTc                                                                                    <  1:3162247/151‑1 (MQ=255)
                                                               aGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCt                                                                                <  1:1434539/151‑1 (MQ=255)
                                                                     gTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGtt                                                                          >  1:2294855/1‑151 (MQ=255)
                                                                          cgTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGa                                                                     >  1:1736757/1‑151 (MQ=255)
                                                                            tATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGAcc                                                                   >  1:1636141/1‑151 (MQ=255)
                                                                                cAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTtat                                                               <  1:332661/151‑1 (MQ=255)
                                                                                   ggTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATAcc                                                            >  1:3310755/1‑151 (MQ=255)
                                                                                   ggTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATAcc                                                            <  1:685565/151‑1 (MQ=255)
                                                                                   ggTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGATTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATAcc                                                            <  1:2626419/151‑1 (MQ=255)
                                                                                    gTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCg                                                           <  1:1471460/151‑1 (MQ=255)
                                                                                    gTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCg                                                           >  1:1964594/1‑151 (MQ=255)
                                                                                     tCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGa                                                          >  1:2327395/1‑151 (MQ=255)
                                                                                          cACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGa                                                     <  1:1724625/151‑1 (MQ=255)
                                                                                            cGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATa                                                   <  1:3436350/151‑1 (MQ=255)
                                                                                            cGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATa                                                   <  1:3207466/151‑1 (MQ=255)
                                                                                             gTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATaa                                                  >  1:1206777/1‑151 (MQ=255)
                                                                                             gTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATaa                                                  >  1:2892669/1‑151 (MQ=255)
                                                                                             gTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATaa                                                  >  1:2703813/1‑151 (MQ=255)
                                                                                               cGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAat                                                >  1:585523/1‑151 (MQ=255)
                                                                                               cGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAat                                                <  1:135990/151‑1 (MQ=255)
                                                                                               cGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAat                                                <  1:1482232/151‑1 (MQ=255)
                                                                                                gATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAata                                               >  1:631532/1‑151 (MQ=255)
                                                                                                  tGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATAtt                                             <  1:1052805/151‑1 (MQ=255)
                                                                                                  tGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATAtt                                             <  1:648181/151‑1 (MQ=255)
                                                                                                  tGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATAtt                                             <  1:1358135/151‑1 (MQ=255)
                                                                                                   gTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATAttt                                            <  1:1475779/151‑1 (MQ=255)
                                                                                                   gTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATAttt                                            <  1:2980148/151‑1 (MQ=255)
                                                                                                    tCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACAGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATAtttt                                           <  1:1425085/151‑1 (MQ=255)
                                                                                                      aaGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATAtttttt                                         <  1:2044820/151‑1 (MQ=255)
                                                                                                        gggTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTcc                                       <  1:2218819/151‑1 (MQ=255)
                                                                                                          gTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCg                                     <  1:1659541/151‑1 (MQ=255)
                                                                                                          gTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCg                                     >  1:2486412/1‑151 (MQ=255)
                                                                                                           ttATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCgg                                    >  1:1444852/1‑151 (MQ=255)
                                                                                                            tATGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGc                                   >  1:2728929/1‑151 (MQ=255)
                                                                                                             aTGTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCa                                  <  1:3168337/151‑1 (MQ=255)
                                                                                                               gTATATGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATa                                <  1:77595/151‑1 (MQ=255)
                                                                                                                   atGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAgg                            >  1:2176977/1‑150 (MQ=255)
                                                                                                                   atGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGt                            >  1:291488/1‑151 (MQ=255)
                                                                                                                   atGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGt                            >  1:1896493/1‑151 (MQ=255)
                                                                                                                    tGGGCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGAATAAAGtc                           >  1:1161811/1‑151 (MQ=255)
                                                                                                                      ggCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGtcaa                         <  1:1041457/151‑1 (MQ=255)
                                                                                                                       gCTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGtcaat                        <  1:1804316/151‑1 (MQ=255)
                                                                                                                        cTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGtcaatc                       >  1:256658/1‑151 (MQ=255)
                                                                                                                        cTTGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGtcaatc                       <  1:493150/151‑1 (MQ=255)
                                                                                                                         ttGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGtcaatca                      <  1:3186093/151‑1 (MQ=255)
                                                                                                                         ttGTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGtcaatca                      <  1:391351/151‑1 (MQ=255)
                                                                                                                           gTCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGTCAATCAAg                    >  1:2961188/1‑151 (MQ=255)
                                                                                                                            tCCTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGTCAATCAAGc                   >  1:3235669/1‑151 (MQ=255)
                                                                                                                             ccTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGTCAATCAAGCg                  >  1:1623915/1‑151 (MQ=255)
                                                                                                                              cTTGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGTCAATCAAGCGt                 >  1:2192777/1‑151 (MQ=255)
                                                                                                                               ttGATATCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGTCAATCAAGCGTc                <  1:492398/151‑1 (MQ=255)
                                                                                                                                  atatCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGTCAATCAAGCGTCTTa             <  1:2274176/151‑1 (MQ=255)
                                                                                                                                  atatCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGTCAATCAAGCGTCTTa             >  1:1516939/1‑151 (MQ=255)
                                                                                                                                   tatCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGTCAATCAAGCGTCTTAc            >  1:2448840/1‑151 (MQ=255)
                                                                                                                                    atCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGTCAATCAAGCGTCTTACa           >  1:2799184/1‑151 (MQ=255)
                                                                                                                                    atCGTCTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGTCAATCAAGCGTCTTACa           >  1:1172130/1‑151 (MQ=255)
                                                                                                                                         cTAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGTCAATCAAGCGTCTTACAGCCGa      >  1:1876054/1‑151 (MQ=255)
                                                                                                                                          tAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGTCAATCAAGCGTCTTACAGCCGaa     >  1:186228/1‑151 (MQ=255)
                                                                                                                                          tAAGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGTCAATCAAGCGTCTTACAGCCGaa     <  1:1036375/151‑1 (MQ=255)
                                                                                                                                           aaGACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGTCAATCAAGCGTCTTACAGCCGAAg    <  1:2789931/151‑1 (MQ=255)
                                                                                                                                             gACATGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGTCAATCAAGCGTCTTACAGCCGAAGCa  <  1:565546/151‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                  
CTTGAACAGATGTATTGAATCCAAAAGTTCCCTGGAGCTGATCCCGCCGGGCTCCGGTGTACCAGTACCGTTCGCGTATGCAGGGTCTACCACGTCGATGTCAAGGGTTATGTATATGGGCTTGTCCTTGATATCGTCTAAGACACGGCTTAACGGCTCAAAAACGTCGATAGTATACATGTTGGTATGTTTTTTTGCATATTCAAACTCGTCTTTCGTTCCGGACCTTATACCGAACTGATAAATATTTTTTCCCGGCATAAAGTCAATCAAGCGTCTTACAGCCGAAGCA  >  CP002416/1785879‑1786170

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑