BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP002416	1,076,195	G→T	V365F (GTT→TTT)	Clo1313_0923 →	aspartyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP002416	1,076,195	0	G	T	99.3%	544.2 / ‑3.8	142	V365F (GTT→TTT)	Clo1313_0923	aspartyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base G (0/1); new base T (68/73); total (68/74)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.94e-01

Only 100 of 143 total aligned reads displayed.

AGGTATTTTCCGATGCCGTCAAAAACGGAGGAAGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATAGTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGCCGACAAAAATGAGGTTGTGTTCGATTCACT  >  CP002416/1076052‑1076340

aGGTATTTTCCGATGCCGTCAAAAACGGAGGAAGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTggg                                                                                                                                             <  1:784976/151‑1 (MQ=255)

     ttttCCGATGCCGTCAAAAACGGAGGAAGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGaaa                                                                                                                                        <  1:1944533/151‑1 (MQ=255)

      tttCCGATGCCGTCAAAAACGGAGGAAGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGaaaa                                                                                                                                       >  1:2103590/1‑151 (MQ=255)

       ttCCGATGCCGTCAAAAACGGAGGAAGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAAc                                                                                                                                      <  1:951437/151‑1 (MQ=255)

           gATGCCGTCAAAAACGGAGGAAGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGc                                                                                                                                  <  1:2528729/151‑1 (MQ=255)

             tGCCGTCAAAAACGGAGGAAGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCAt                                                                                                                                >  1:1181511/1‑151 (MQ=255)

                cGTCAAAAACGGAGGAAGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATaaa                                                                                                                             >  1:3251695/1‑151 (MQ=255)

                cGTCAAAAACGGAGGAAGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATaaa                                                                                                                             >  1:3035843/1‑151 (MQ=255)

                 gTCAAAAACGGAGGAAGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAAt                                                                                                                            >  1:436720/1‑151 (MQ=255)

                 gTCAAAAACGGAGGAAGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAAt                                                                                                                            >  1:2671762/1‑151 (MQ=255)

                   cAAAAACGGAGGAAGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGCGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATcc                                                                                                                          >  1:1038988/1‑151 (MQ=255)

                    aaaaaCGGAGGAAGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATccc                                                                                                                         <  1:234291/151‑1 (MQ=255)

                      aaaCGGAGGAAGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCg                                                                                                                       <  1:2513203/151‑1 (MQ=255)

                      aaaCGGAGGAAGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCg                                                                                                                       <  1:438931/151‑1 (MQ=255)

                        aCGGAGGAAGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAATCTATGGCGCAATGGGAATGGCCTGGATATTTGCGGGAGAAAACGAGCATAAATCCCCGA‑t                                                                                                                    >  1:2768734/1‑151 (MQ=255)

                          ggaggaAGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTa                                                                                                                  >  1:2487605/1‑151 (MQ=255)

                          ggaggaAGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTa                                                                                                                  <  1:1872369/151‑1 (MQ=255)

                                aGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAAt                                                                                                            >  1:826132/1‑151 (MQ=255)

                                   gTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCt                                                                                                         <  1:965926/151‑1 (MQ=255)

                                     tCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCttt                                                                                                       <  1:935770/151‑1 (MQ=255)

                                        ggCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTAcc                                                                                                    >  1:3350787/1‑151 (MQ=255)

                                          cGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGa                                                                                                  <  1:2956681/151‑1 (MQ=255)

                                          cGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGa                                                                                                  >  1:3020693/1‑151 (MQ=255)

                                          cGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGa                                                                                                  <  1:1980048/151‑1 (MQ=255)

                                           gATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAg                                                                                                 <  1:2958992/151‑1 (MQ=255)

                                            aTAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAgg                                                                                                >  1:1542296/1‑151 (MQ=255)

                                             tAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGa                                                                                               <  1:673592/151‑1 (MQ=255)

                                              aaaTGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGAc                                                                                              >  1:1614776/1‑151 (MQ=255)

                                              aaaTGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGAc                                                                                              <  1:2376732/151‑1 (MQ=255)

                                              aaaTGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGAc                                                                                              <  1:2187422/151‑1 (MQ=255)

                                               aaTGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACg                                                                                             <  1:134428/151‑1 (MQ=255)

                                                 tGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGaa                                                                                           <  1:1235121/151‑1 (MQ=255)

                                                   ccAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAAt                                                                                         <  1:2589193/151‑1 (MQ=255)

                                                     aaaGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCa                                                                                       <  1:2409074/151‑1 (MQ=255)

                                                      aaGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCaa                                                                                      <  1:668026/151‑1 (MQ=255)

                                                       aGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAg                                                                                     <  1:3109825/151‑1 (MQ=255)

                                                         gATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGc                                                                                   <  1:131659/151‑1 (MQ=255)

                                                           tgtgGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCg                                                                                 <  1:1228175/151‑1 (MQ=255)

                                                             tgGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGtt                                                                               >  1:1555296/1‑151 (MQ=255)

                                                             tgGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGtt                                                                               >  1:1108371/1‑151 (MQ=255)

                                                              ggAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGttt                                                                              >  1:604997/1‑151 (MQ=255)

                                                                  ataaatTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGAc                                                                          >  1:407283/1‑151 (MQ=255)

                                                                    aaatTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACaa                                                                        >  1:3297786/1‑151 (MQ=255)

                                                                    aaatTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACaa                                                                        >  1:2114229/1‑151 (MQ=255)

                                                                    aaatTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACaa                                                                        >  1:1045392/1‑151 (MQ=255)

                                                                    aaatTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACaa                                                                        >  1:927997/1‑151 (MQ=255)

                                                                      atTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGa                                                                      <  1:175/151‑1 (MQ=255)

                                                                        tCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAAt                                                                    <  1:3197952/151‑1 (MQ=255)

                                                                         cagcagAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATg                                                                   >  1:3072019/1‑151 (MQ=255)

                                                                            cagAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCGGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCgg                                                                >  1:2833632/1‑151 (MQ=255)

                                                                               aaaGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCa                                                             <  1:1205954/151‑1 (MQ=255)

                                                                               aaaGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCa                                                             <  1:1717137/151‑1 (MQ=255)

                                                                                 aGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGa                                                           <  1:1440257/151‑1 (MQ=255)

                                                                                   gAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAAc                                                         <  1:116202/151‑1 (MQ=255)

                                                                                    aaaTAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAAcc                                                        >  1:3428000/1‑151 (MQ=255)

                                                                                     aaTAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAAccc                                                       >  1:2674631/1‑151 (MQ=255)

                                                                                     aaTAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAAccc                                                       >  1:3193476/1‑151 (MQ=255)

                                                                                      aTAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCg                                                      >  1:1253543/1‑151 (MQ=255)

                                                                                      aTAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCg                                                      >  1:2602257/1‑151 (MQ=255)

                                                                                      aTAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCg                                                      <  1:1403423/151‑1 (MQ=255)

                                                                                      aTAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCg                                                      >  1:790757/1‑151 (MQ=255)

                                                                                      aTAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCg                                                      <  1:483641/151‑1 (MQ=255)

                                                                                       tAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCgg                                                     <  1:2446701/151‑1 (MQ=255)

                                                                                        aGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGga                                                    >  1:2184424/1‑151 (MQ=255)

                                                                                        aGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGga                                                    <  1:2017339/151‑1 (MQ=255)

                                                                                            gCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGAcc                                                >  1:3019712/1‑151 (MQ=255)

                                                                                             cccTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCt                                               >  1:1336412/1‑151 (MQ=255)

                                                                                              ccTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTc                                              <  1:226996/151‑1 (MQ=255)

                                                                                              ccTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTc                                              >  1:2114170/1‑151 (MQ=255)

                                                                                                ttGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCat                                            >  1:2311988/1‑151 (MQ=255)

                                                                                                ttGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCat                                            <  1:2459974/151‑1 (MQ=255)

                                                                                                 tGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCata                                           <  1:899759/151‑1 (MQ=255)

                                                                                                  ggTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCatat                                          <  1:1661865/151‑1 (MQ=255)

                                                                                                  ggTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCatat                                          <  1:3194401/151‑1 (MQ=255)

                                                                                                   gTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATg                                         >  1:761406/1‑151 (MQ=255)

                                                                                                    tGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGc                                        >  1:2691717/1‑151 (MQ=255)

                                                                                                    tGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGc                                        <  1:2430873/151‑1 (MQ=255)

                                                                                                     gAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCt                                       >  1:3163461/1‑151 (MQ=255)

                                                                                                      aaTTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCtt                                      >  1:1155168/1‑151 (MQ=255)

                                                                                                          cGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTAtt                                  <  1:2103591/151‑1 (MQ=255)

                                                                                                          cGTAAAAACCTATGGAGCAAAGGGAAAGGCCTGGATAGTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTAtt                                  <  1:385169/151‑1 (MQ=255)

                                                                                                            tAAAAACCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGc                                >  1:569823/1‑151 (MQ=255)

                                                                                                             aaaaaCCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGcc                               >  1:541864/1‑151 (MQ=255)

                                                                                                              aaaaCCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGCCg                              >  1:1181750/1‑151 (MQ=255)

                                                                                                              aaaaCCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGCCg                              <  1:2321086/151‑1 (MQ=255)

                                                                                                               aaaCCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTGCCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGCCGa                             <  1:1116665/151‑1 (MQ=255)

                                                                                                               aaaCCTATGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGCCGa                             <  1:690760/151‑1 (MQ=255)

                                                                                                                     aTGGTGCAAAGGGAATGGCCTGTATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGCCGACaaaaa                       <  1:575599/151‑1 (MQ=255)

                                                                                                                     aTGGTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGCCGACaaaaa                       >  1:1407490/1‑151 (MQ=255)

                                                                                                                       ggTGCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGCCGACAAAAATg                     <  1:2742695/151‑1 (MQ=255)

                                                                                                                          gCAAAGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGCCGACAAAAATGAgg                  <  1:2562494/151‑1 (MQ=255)

                                                                                                                            aaaGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGCCGACAAAAATGAGGtt                <  1:3051847/151‑1 (MQ=255)

                                                                                                                             aaGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGCTGACAAAAATGAGGTtg               >  1:1456685/1‑151 (MQ=255)

                                                                                                                             aaGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGCCGACAAAAATGAGGTtg               >  1:2841402/1‑151 (MQ=255)

                                                                                                                              aGGGAATGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGCCGACAAAAATGAGGTtgt              >  1:1032606/1‑151 (MQ=255)

                                                                                                                                  aaTGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGCCGACAAAAATGAGGTTGTGTTc          <  1:2175920/151‑1 (MQ=255)

                                                                                                                                   aTGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGCCGACAAAAATGAGGTTGTGTTCg         <  1:589183/151‑1 (MQ=255)

                                                                                                                                    tGGCCTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGCCGACAAAAATGAGGTTGTGTTCGa        >  1:1301297/1‑151 (MQ=255)

                                                                                                                                       ccTGGATATTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGCCGACAAAAATGAGGTTGTGTTCGATTc     >  1:2374694/1‑151 (MQ=255)

                                                                                                                                          ggATATTTGTGGGATAAAACGAACCTAAATCCCCGAATTCCCAAAT‑CTTTTCCCAGGACGAAAACAAAGCCCTTTTGGCCAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGCCGACAAAAATGAGGTTGTGTTCGATTCACt  <  1:328792/151‑1 (MQ=255)

AGGTATTTTCCGATGCCGTCAAAAACGGAGGAAGCGTTCGGGCGATAAATGCCAAAGGATGTGGAAATAAATTCAGCAGAAAGGAAATAGATGCCCTTGGTGAATTCGTAAAAACCTATGGTGCAAAGGGAATGGCCTGGATAGTTGTGGGAGAAAACGAGCATAAATCCCCGA‑TTACCAAATTCTTTACCGAGGACGAAATCAAGGCCGTTTTGACAAGAATGCGGGCAGAACCCGGAGACCTCATATGCTTTATTGCCGACAAAAATGAGGTTGTGTTCGATTCACT  >  CP002416/1076052‑1076340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 38 ≤` `ATCG/ATCG` `< 39 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`