Predicted mutation
evidence seq id position mutation annotation gene description
RA CP002416 677,297 A→G intergenic (+941/+1706) Clo1313_0606 → / ← Clo1313_0609 hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP002416677,2970AG99.4% 629.1 / NA 170intergenic (+941/+1706)Clo1313_0606/Clo1313_0609hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (82/87);  total (83/87)
Fisher's exact test for biased strand distribution p-value = 4.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.88e-01

Only 100 of 170 total aligned reads displayed.

GAAAATGTTGTGGATATCGGTAAAAGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGAACAATTATGATAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATGGATCTGTTATCTATAATAATGCAATTGCAAATGCACAC  >  CP002416/677155‑677443
                                                                                                                                              |                                                                                                                                                  
  aaaTGTTGTGGATATCGGTAAAAGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATAtttt                                                                                                                                          <  1:828086/151‑1 (MQ=255)
    aTGTTGTGGATATCGGTAAAAGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTa                                                                                                                                        >  1:3165223/1‑151 (MQ=255)
      gttgtGGATATCGGTAAAAGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACg                                                                                                                                      <  1:1082154/151‑1 (MQ=255)
      gttgtGGATATCGGTAAAAGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACg                                                                                                                                      <  1:843824/151‑1 (MQ=255)
       ttgtGGATATCGGTAAAAGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGc                                                                                                                                     <  1:2930874/151‑1 (MQ=255)
        tgtgGATATCGGTAAAAGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCa                                                                                                                                    <  1:1037834/151‑1 (MQ=255)
        tgtgGATATCGGTAAAAGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCa                                                                                                                                    <  1:2027809/151‑1 (MQ=255)
          tgGATATCGGTAAAAGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATc                                                                                                                                  >  1:3069041/1‑151 (MQ=255)
             atatCGGTAAAAGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCaaa                                                                                                                               >  1:2916112/1‑151 (MQ=255)
              tatCGGTAAAAGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAAt                                                                                                                              <  1:2623267/151‑1 (MQ=255)
              tatCGGTAAAAGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAAt                                                                                                                              <  1:3390512/151‑1 (MQ=255)
              tatCGGTAAAAGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAAt                                                                                                                              <  1:708526/151‑1 (MQ=255)
                 cGGTAAAAGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACt                                                                                                                           >  1:2080627/1‑151 (MQ=255)
                 cGGTAAAAGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACt                                                                                                                           >  1:2464672/1‑151 (MQ=255)
                   gTAAAAGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTg                                                                                                                         <  1:182638/151‑1 (MQ=255)
                    tAAAAGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGa                                                                                                                        >  1:1994615/1‑151 (MQ=255)
                    tAAAAGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGa                                                                                                                        <  1:3077924/151‑1 (MQ=255)
                     aaaaGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATGTTTTTACGCATCAAATACTTGat                                                                                                                       <  1:726576/151‑1 (MQ=255)
                     aaaaGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGat                                                                                                                       >  1:1612808/1‑151 (MQ=255)
                       aaGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGatat                                                                                                                     <  1:293824/151‑1 (MQ=255)
                         gTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGa                                                                                                                   >  1:848568/1‑151 (MQ=255)
                         gTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGa                                                                                                                   >  1:535117/1‑151 (MQ=255)
                           aCAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGAtt                                                                                                                 <  1:3145708/151‑1 (MQ=255)
                                 tGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCa                                                                                                           >  1:1573094/1‑151 (MQ=255)
                                   cAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAg                                                                                                         >  1:139281/1‑151 (MQ=255)
                                       ctctTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGAt                                                                                                     <  1:593745/151‑1 (MQ=255)
                                          ttAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATTCTTGATATGATTGACTCAAGGGATTAt                                                                                                  >  1:2363466/1‑151 (MQ=255)
                                          ttAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTAt                                                                                                  >  1:2464650/1‑151 (MQ=255)
                                          ttAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTAt                                                                                                  <  1:1062757/151‑1 (MQ=255)
                                           tAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATg                                                                                                 >  1:1108399/1‑151 (MQ=255)
                                            aaaaaaaaGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATga                                                                                                <  1:2302206/151‑1 (MQ=255)
                                            aaaaaaaaGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATga                                                                                                >  1:1139249/1‑151 (MQ=255)
                                            aaaaaaaaGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATga                                                                                                >  1:953278/1‑151 (MQ=255)
                                              aaaaaaGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATgaga                                                                                              >  1:2110908/1‑151 (MQ=255)
                                                aaaaGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACt                                                                                            <  1:1003131/151‑1 (MQ=255)
                                                   aGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGtt                                                                                         <  1:1647137/151‑1 (MQ=255)
                                                   aGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGtt                                                                                         <  1:1496671/151‑1 (MQ=255)
                                                   aGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGtt                                                                                         >  1:2725427/1‑151 (MQ=255)
                                                      aaCACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGAGAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGc                                                                                      <  1:3386133/151‑1 (MQ=255)
                                                      aaCACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGc                                                                                      >  1:1006321/1‑151 (MQ=255)
                                                        cacTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGa                                                                                    >  1:1375390/1‑151 (MQ=255)
                                                         acTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAg                                                                                   <  1:3233412/151‑1 (MQ=19)
                                                          cTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGt                                                                                  <  1:975363/151‑1 (MQ=16)
                                                          cTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGt                                                                                  <  1:2283786/151‑1 (MQ=16)
                                                          cTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGt                                                                                  >  1:1098/1‑151 (MQ=16)
                                                              ttgttgACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGtt                                                                              <  1:3069042/151‑1 (MQ=12)
                                                                gttgACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTaa                                                                            >  1:1322236/1‑151 (MQ=12)
                                                                 ttgACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTAAGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTaaa                                                                           >  1:3277034/1‑151 (MQ=255)
                                                                  tgACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTaaaa                                                                          >  1:3356085/1‑151 (MQ=12)
                                                                  tgACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTaaaa                                                                          >  1:729877/1‑151 (MQ=12)
                                                                      aaaaaGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACat                                                                      <  1:2552383/151‑1 (MQ=12)
                                                                      aaaaaGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACat                                                                      <  1:2939120/151‑1 (MQ=12)
                                                                      aaaaaGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACat                                                                      >  1:1938103/1‑151 (MQ=12)
                                                                        aaaGACAGTAACAGTTGTTTGGATTGAAGATTTTGCTAATAAAAAGCGAAAAAGCTATGGGAAAAATATGGTAGATTTTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACatat                                                                    <  1:1212036/151‑1 (MQ=11)
                                                                        aaaGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACatat                                                                    <  1:1593880/151‑1 (MQ=12)
                                                                        aaaGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACatat                                                                    >  1:2417327/1‑151 (MQ=12)
                                                                        aaaGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACatat                                                                    >  1:3314136/1‑151 (MQ=12)
                                                                        aaaGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGAACAATTATGATAGTTATTTTTATGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTCAAAACatat                                                                    >  1:2355269/1‑151 (MQ=255)
                                                                         aaGACAGTAACAGTTGTTTGCATTGTTGATTTAGCTATTAAAAATCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCATGGGATTATGAGACTGTTTGTGAGTGGCTAAAAACATATc                                                                   >  1:1892630/1‑151 (MQ=11)
                                                                         aaGACAGTAACAGTTGTTTGCATTGATGATTTCGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATc                                                                   <  1:1259105/151‑1 (MQ=255)
                                                                          aGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATcc                                                                  >  1:2650849/1‑151 (MQ=16)
                                                                            aCAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCaa                                                                >  1:2671282/1‑151 (MQ=19)
                                                                             cAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCaaa                                                               >  1:2662649/1‑151 (MQ=255)
                                                                              aGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAAt                                                              >  1:1193913/1‑151 (MQ=255)
                                                                              aGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAAt                                                              >  1:875116/1‑151 (MQ=255)
                                                                                tAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGAGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCt                                                            <  1:215734/151‑1 (MQ=255)
                                                                                 aaCAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCtt                                                           <  1:726782/151‑1 (MQ=255)
                                                                                  aCAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTa                                                          >  1:442833/1‑151 (MQ=255)
                                                                                   cAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAg                                                         >  1:105451/1‑151 (MQ=255)
                                                                                   cAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAg                                                         >  1:904070/1‑151 (MQ=255)
                                                                                       tgttTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGtgtg                                                     >  1:1801271/1‑151 (MQ=255)
                                                                                        gttTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGa                                                    <  1:804692/151‑1 (MQ=255)
                                                                                           tGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGatat                                                 <  1:1212710/151‑1 (MQ=255)
                                                                                           tGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGatat                                                 >  1:1233887/1‑151 (MQ=255)
                                                                                           tGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGatat                                                 <  1:1734488/151‑1 (MQ=255)
                                                                                            gCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATc                                                >  1:2115508/1‑151 (MQ=255)
                                                                                            gCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATc                                                <  1:811425/151‑1 (MQ=255)
                                                                                             cATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCa                                               >  1:20754/1‑151 (MQ=255)
                                                                                              aTTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCaa                                              >  1:1297959/1‑151 (MQ=255)
                                                                                                 gatgatTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAagag                                           >  1:1869101/1‑151 (MQ=255)
                                                                                                 gatgatTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCACCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGAAATCAagag                                           <  1:1420602/151‑1 (MQ=11)
                                                                                                    gatTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATg                                        <  1:738219/151‑1 (MQ=255)
                                                                                                    gatTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATg                                        >  1:603731/1‑151 (MQ=255)
                                                                                                     atTTTGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATgg                                       >  1:2341613/1‑151 (MQ=255)
                                                                                                       tttGCTATTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATGGAt                                     <  1:2501557/151‑1 (MQ=255)
                                                                                                             aTTAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATGGATCTGTTa                               <  1:3333966/151‑1 (MQ=255)
                                                                                                               tAAAAAGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATGGATCTGTTATc                             >  1:1658481/1‑151 (MQ=255)
                                                                                                                 aaaaGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATGGATCTGTTATCta                           <  1:90487/151‑1 (MQ=255)
                                                                                                                    aGCGAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATGGATCTGTTATCTataa                        <  1:2214373/151‑1 (MQ=255)
                                                                                                                       gAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATGGATCTGTTATCTAtaataa                     >  1:2985854/1‑151 (MQ=255)
                                                                                                                       gAAAAAGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATGGATCTGTTATCTAtaataa                     >  1:426574/1‑151 (MQ=255)
                                                                                                                        aaaaaGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATGGATCTGTTATCTATaataat                    >  1:457087/1‑151 (MQ=255)
                                                                                                                        aaaaaGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATGGATCTGTTATCTATaataat                    >  1:3339242/1‑151 (MQ=255)
                                                                                                                        aaaaaGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATGGATCTGTTATCTATaataat                    <  1:3247370/151‑1 (MQ=255)
                                                                                                                         aaaaGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATGGATCTGTTATCTATAATAATg                   <  1:2602693/151‑1 (MQ=255)
                                                                                                                           aaGCTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATGGATCTGTTATCTATAATAATGCa                 <  1:1936223/151‑1 (MQ=255)
                                                                                                                              cTATGGGATAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATGGATCTGTTATCTATAATAATGCAAtt              >  1:3469746/1‑151 (MQ=255)
                                                                                                                              cTATGGGACAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATGGATCTGTTATCTATAATAATGCAAtt              <  1:2692798/151‑1 (MQ=255)
                                                                                                                                     aCAATTATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATGGATCTGTTATCTATAATAATGCAATTGCAAATg       >  1:3222981/1‑151 (MQ=25)
                                                                                                                                         ttATGGTAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTCAGTGTGATATCAAGAGATGGATCTGTTATCTATAATAATGCAATTGCAAATGcaca   >  1:2744465/1‑151 (MQ=255)
                                                                                                                                              |                                                                                                                                                  
GAAAATGTTGTGGATATCGGTAAAAGTACAGTTTGCAATCTCTTAAAAAAAAGAAACACTGGTTGTTGACAAAAAGACAGTAACAGTTGTTTGCATTGATGATTTTGCTATTAAAAAGCGAAAAAGCTATGGAACAATTATGATAGATATTTTTACGCATCAAATACTTGATATGATTGACTCAAGGGATTATGAGACTGTTTGCGAGTGGTTAAAAACATATCCAAATCTTAGTGTGATATCAAGAGATGGATCTGTTATCTATAATAATGCAATTGCAAATGCACAC  >  CP002416/677155‑677443

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑