Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0024162,884,3370GA33.9% 79.5 / 21.3 56E24K (GAA→AAA) Clo1313_2469Prephenate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (26/11);  new base A (2/17);  total (28/28)
Fisher's exact test for biased strand distribution p-value = 4.00e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

ACAACTTCCGCCGGAAGATTTCTGTGAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGCG‑AAA‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACATTATTTTCATATGTACACCGGTT  >  CP002416/2884188‑2884486
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acaacTTCCGCCGGAAGATTTCTGTGAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGCG‑AAA‑GCTTTgaaaga                                                                                                                                                           <  1:1359930/151‑1 (MQ=255)
  aacTTCCGCCGGAAGATTTCTGTGAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGCG‑AAA‑GCTTTgaaagaaa                                                                                                                                                         <  1:94655/151‑1 (MQ=255)
    cTTCCGCCGGAAGATTTCTGTGAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGCG‑AAA‑GCTTTGAAAGAAAA‑g                                                                                                                                                      <  1:1197705/151‑1 (MQ=255)
     ttCCGCCGGAAGATTTCTGTGAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGCG‑AAA‑GCTTTGAAAGAAAA‑G‑c                                                                                                                                                    <  1:2210583/151‑1 (MQ=255)
      tCCGCCGGAAGATTTCTGTGAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGGCGCTGGCG‑GAA‑GCTTTGAAAGaaaaact                                                                                                                                                     >  1:2056321/1‑148 (MQ=255)
        cgccgGAAGATTTCTGTGAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTGGCTGGGG‑GAA‑GCATTGTAAGAAAA‑gactg                                                                                                                                                  >  1:1677894/1‑147 (MQ=255)
        cgccgGAAGATTTCTGTGAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGCG‑AAA‑GCTTTGAAAGAAAA‑G‑CTTg                                                                                                                                                 <  1:1558766/151‑1 (MQ=255)
          ccgGAAGATTTCTGTGAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGGGGCTGGGG‑AAA‑GCTTTGTAAGaaaaagttggg                                                                                                                                                 >  1:3209080/1‑144 (MQ=255)
           cgGAAGATTTCTGTGAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGCG‑AAA‑GCTTTGAAAGAAAA‑G‑CTTGG‑Ca                                                                                                                                             <  1:1042124/151‑1 (MQ=255)
              aaGATTTCTGTGAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGCG‑AAA‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTg                                                                                                                                          <  1:325310/151‑1 (MQ=255)
               aGATTTCTGTGAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGGGGCTGGCG‑AAA‑GCTTTTTAAGAAAA‑gaatggccttgt                                                                                                                                           >  1:1058574/1‑140 (MQ=255)
                   ttCTGTGAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGGTGGGG‑GAA‑GCATTTAAAGAAAA‑A‑CTTGG‑GATTG‑AGtga                                                                                                                                    >  1:3023639/1‑149 (MQ=255)
                    tCTGTGAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGGGGCCGGCG‑GAA‑GCTTTTTAAGAAAA‑A‑CTTGG‑GATTgtgtgca                                                                                                                                    >  1:2281156/1‑145 (MQ=255)
                    tCTGTGAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGGCGCTGGCG‑GAA‑GATTTTAAAGAAAA‑A‑ATTGG‑GATTG‑AGGCaa                                                                                                                                   >  1:2187614/1‑151 (MQ=255)
                      tgtgAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTTGCTGGCG‑GAA‑GCCTTTTAAGAAAA‑A‑ATTGG‑CCTTG‑TGTCAATa                                                                                                                                 >  1:1890588/1‑151 (MQ=255)
                      tgtgAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGCG‑AAA‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATa                                                                                                                                 <  1:3317480/151‑1 (MQ=255)
                       gtgAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGCG‑AAA‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATaa                                                                                                                                <  1:974049/151‑1 (MQ=255)
                       gtgAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGGGGCTGGGG‑AAA‑GGTTTGAAAGAAAA‑A‑GTTGG‑Caattggtctataa                                                                                                                                 >  1:2935493/1‑139 (MQ=255)
                       gtgAACTGTATAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGCG‑AAA‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATaa                                                                                                                                <  1:982651/151‑1 (MQ=255)
                        tgAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGCG‑GAA‑GATTTTAAAAAAAA‑A‑CTTGG‑CGTTG‑Aggtactaaa                                                                                                                               >  1:1976571/1‑143 (MQ=255)
                         gAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGCG‑AAA‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATAAcc                                                                                                                              <  1:1003954/151‑1 (MQ=255)
                          aaCTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGGCGCTGGGG‑GAA‑ACTTTTAAAAAAAA‑A‑CTTTG‑CATTG‑TGTCAAAAAccg                                                                                                                             >  1:3003751/1‑151 (MQ=255)
                                gtAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGGG‑GAA‑GCTTTTTAAGAAAA‑A‑CCTGG‑GATTG‑AGTCAATAACCGCCGTTg                                                                                                                       >  1:158041/1‑151 (MQ=255)
                                    aGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGGG‑GAA‑GCTTTTTAAGAAAA‑G‑ACTGG‑CATTG‑GGTGAATaaacccccttggcaa                                                                                                                   >  1:1846839/1‑138 (MQ=255)
                                        ataAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGGGGCCGGCG‑AAA‑ACTTTTAAAGAAAA‑A‑CCTGG‑GCTTG‑AGTCAATAAACGCCGGTGACATCAAt                                                                                                               >  1:3010790/1‑151 (MQ=255)
                                                  ttGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGGCGCTGGCG‑GAA‑GCTTTTAAAGAAAA‑G‑CTTGG‑GATTG‑AGTCAATAAACGCCGGTGACATCAATGAGAAAAgac                                                                                                     >  1:2704807/1‑149 (MQ=255)
                                                      caaaaTATTAGGAG‑GAGATTTTCCTTATGCAGGTAAAAAAAATATCATTTTTCGGGTTTGG‑ACTTACGGGGGGGTCGCGGGCG‑AAA‑GCTTTAAAAAAAAA‑G‑CTTGG‑CTTTG‑ATTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAg                                                                                                 <  1:2708179/149‑1 (MQ=255)
                                                       aaaaTATTAGAAA‑GAGATTTTCACTATGCGGGTTAAAAAAATATCCATTATCGGGCTGGG‑ACTTATCGGCGGGTCGTGGGAG‑AAA‑GTTTTAAAAAAAAA‑G‑CT‑GG‑GATTGGAGTAAAAAACCGCCGTTGACATCAATGAGAAAAGTCTGAGc                                                                                                <  1:2847523/150‑1 (MQ=255)
                                                        aaaTATAAGGAA‑GAGATTTTCCCTATGCAGGTTAAAAAGATATCCATTAGGGGGTTTGG‑ATTTATGGGGGGGTCGCGGGCG‑AAA‑GTTTTAAAAAAAAA‑G‑TTGGG‑CATTG‑AGTAAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGcc                                                                                               <  1:3008570/151‑1 (MQ=255)
                                                         aaatttAGGAAAGAGAGTTTCACTAGGAAGGTAAAAAAAATCTCCATTATCGGGTTGGG‑TTTTATGGGGGGGTCGCGGGCG‑AAA‑GTTTAAAAAAAAAA‑G‑TTGGG‑CTTTG‑ATTAAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGcc                                                                                               <  1:1590349/147‑1 (MQ=255)
                                                          atatTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGCCGCTGGCG‑GAA‑GCTTTTAAAGAAAA‑A‑ACTGG‑GATTG‑AGTCAATAAACCCCCCTGGCATCAAAGAGAAAAATCTGAGcccg                                                                                             >  1:261897/1‑149 (MQ=255)
                                                          atatTAGAAA‑GAGATTTCCACTATGGGGGTAAAAAAGATATCCATTAGGGGGTTGGG‑ATTTACCGGGGGGTCGCGGGCA‑AAA‑GCTTT‑AAAAAAAA‑G‑GTTGGGCTTTG‑AGTAAAAAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAg                                                                                             <  1:1677893/151‑1 (MQ=255)
                                                           tatTAGGAA‑GGGAGTTCCACTATGCAGTTTAAAAAGATATCCATTAGGGGGCTGGG‑CTTTATGGGGGGGTCGCGGGC‑‑AAA‑GTTTTTAAAAAAAA‑GGCTTGG‑CTTTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAgg                                                                                            <  1:219622/151‑1 (MQ=255)
                                                             ttAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTGGCCGGCG‑GAA‑GCCTTTAAAGAAAA‑A‑CTTGG‑CGTTG‑AGGCAATAAACCCCGCTGTCATCAAAGAGGAAAATCTGGGGCAGGcc                                                                                          >  1:2515669/1‑150 (MQ=255)
                                                              tAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGCG‑AAA‑GCGTTTTAAGAAAA‑A‑CTTTG‑GGTTG‑TGTCAAAAAACACCGTTGACATCCAAGAGAAAAAACTGAGcccagccc                                                                                         >  1:3151395/1‑145 (MQ=255)
                                                                ggAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGCG‑AAA‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCtt                                                                                       >  1:2504272/1‑151 (MQ=255)
                                                                     gagatTTTCACTATGCGGGTTAAAAAAATCTCCATTATCGGGTTTGG‑TTTTACCGGGGGGTCGGTGGCG‑AAA‑GTTTTAAAAAAAAA‑G‑CTTGC‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAag                                                                                   <  1:3436748/151‑1 (MQ=255)
                                                                      agttttacACTATGCAGTTTGAAAAGATACCCTTTA‑CGGGGTTGGCATTTATGGGGGGGCCGCTGG‑G‑AAATGCTTTGAAAAAAAA‑G‑TTTGC‑CATAG‑ATTAAATATCCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAaga                                                                                  <  1:1290050/144‑1 (MQ=255)
                                                                              aCTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGCG‑GAA‑GCTTTTTAAAAAAA‑A‑CTTTG‑CCTTG‑AGTCAATAAACCCCCTTGACATCAATGAGAAAAATCTTTGCCAGGCCCTTTAAAAGGG‑gtttt                                                                         >  1:1134708/1‑150 (MQ=255)
                                                                               cTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGGCGCTGGCG‑GAA‑GCTTTTGAAGAAAA‑A‑ATTGG‑GATTG‑AGTCAATAAACGCCCGTGACATCAATGAGAAAAGAATGAGCCAGGGTCCTAAAGAGGG‑gttttt                                                                        >  1:2213754/1‑149 (MQ=255)
                                                                                tATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGCG‑GAA‑GCTTTTAAAGAAAA‑A‑CTTGG‑CATTG‑AGTCAATAAACGCCGGTGACATCAATGAGAAAAAGATGAGCCAGGCGCTTTAAGAGGG‑GTTtata                                                                       >  1:2399049/1‑151 (MQ=255)
                                                                                    gaGGTTAAAAAGATATCCATTCGCGGGCTTGG‑CCTTATGGGGGGGTCGGGGGCA‑AAA‑G‑TTTTAAAAAAAA‑G‑GCTGG‑GATTGGAGTAAAAAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAaag                                                                   <  1:1717557/150‑1 (MQ=255)
                                                                                    cAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGCG‑AAA‑GCTTTTAAAGAAAA‑A‑CTTGG‑CATTG‑AGGCCATAAACCCCGTTGACATCAATGAGAAAAAGCTGAGCCAGGCGCTTAAAGAGGG‑GTTTATaaaaa                                                                   >  1:395432/1‑150 (MQ=255)
                                                                                       gTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGGCGCCGGCG‑AAA‑GCTTTTTAAGAAAA‑A‑ATTTG‑GATTT‑AGTGAATAAACGCCGGTGACATCAATGAGAAAAGACTGCGCCAGGCGCTTTAAAAGGG‑GGTTTTAAaagaag                                                                >  1:251946/1‑151 (MQ=255)
                                                                                           aaaaGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGGCGCCGGCG‑GAA‑GCTTTGGAAAAAAA‑A‑ATTGG‑CATTT‑AGTCAATAAACGCCCTTTACATTAATAAGAAAAGACTGAGCCAGGCGCCTTAAGAGGG‑TTTTATAAAAAAAgggtt                                                            >  1:974784/1‑148 (MQ=255)
                                                                                           aaaaGATATCCATTAGCGGGCTTGG‑ATTTAGCGGCGGGTCGCGGGCG‑AAA‑GTTTTAAAAAAAAA‑G‑CTTGG‑CATTG‑AGTAAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGttt                                                            <  1:2659385/151‑1 (MQ=255)
                                                                                              aGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGGCGCTGGCG‑AAA‑ACTTTTAAAGAAAA‑A‑CTTGG‑GATTG‑AGGCAATAAACGCCGTTGACATCAATGAGAAAAAGCTTAGCCAGGGTCTTAAAGAGGG‑GTTTATAAAAAAAGGGTTTaa                                                         >  1:842270/1‑150 (MQ=255)
                                                                                                   tCCATTATGGGGCTTGG‑ACTTAGCGGGGGGCCGCGGGCG‑AAA‑GTTTTGAAAAAAAA‑G‑CTTGG‑CATTG‑AGTAAAAAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAAc                                                    <  1:94783/151‑1 (MQ=255)
                                                                                                    ccatgagggggtttGG‑ATTTAGCGGGGGGTCGCGGGCA‑AAA‑GTTTAAAAAAAAAA‑G‑CTTGG‑CATGG‑AGAAAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACt                                                   <  1:1846825/139‑1 (MQ=255)
                                                                                                      aTTATCGGGCTTGG‑ACTTATCGGCGGGGCGCTGGCG‑AAA‑GCTTTTAAAGAAAA‑A‑AATGG‑CGTTG‑AGTCAATAAACGCCGCTGACATCAAAGAGAAAAGACTGAGCCCGGCTCTTTAAGAGGG‑GTTTTTAAAAAAAGGGTTTACCGAACTTa                                                 >  1:2545096/1‑151 (MQ=255)
                                                                                                        tatggggCTGGG‑ACTTATCGGGGGGTCGCTGGCG‑AAA‑GTTTTAAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAAc                                               <  1:620645/147‑1 (MQ=255)
                                                                                                         aTCGGGCTTGG‑ACTTATCGGCGGGGCGCTGGGGGAAA‑ATTTTGAA‑GAAAA‑A‑ATTTG‑GATTG‑AGGTAATAAACCCCGGTGACAACAATGAGAAAAAACTGAGGCAGGGTCTTTAAGAGGGGTTTTTAAAAAAAAGGTTTTAC‑GAACTTaaag                                              >  1:2585133/1‑149 (MQ=255)
                                                                                                                   ggtcttctCGGCGGGTCTCTGGCA‑AAA‑GCTTTAAAAGAAAA‑G‑TTTGG‑CATTG‑AGTAAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGtat                                     <  1:995688/144‑1 (MQ=255)
                                                                                                                           gggcgggccgggggCG‑AAA‑G‑TTTGAAAAAAAAAG‑CTTGG‑CTTTG‑ATTAAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCt                             <  1:2888839/139‑1 (MQ=255)
                                                                                                                           cggggggTCGCGGGAG‑AAA‑GCTTAAAAAAAAAT‑G‑CTTGG‑ATTTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCt                             <  1:364914/147‑1 (MQ=255)
                                                                                                                           cggcggGTCGCTGGCG‑AAA‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCt                             >  1:1577658/1‑151 (MQ=255)
                                                                                                                                  gtcgggggcaaaa‑GTTTTAAAAAAAAA‑G‑CTTGG‑CATGG‑ATTAAAAAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACatt                       <  1:3209079/141‑1 (MQ=255)
                                                                                                                                   cGCTGGCG‑AAA‑GCTTTAAAAAAAAA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACattat                     <  1:883938/151‑1 (MQ=255)
                                                                                                                                    gCTGGCG‑AAA‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACattatt                    >  1:331718/1‑151 (MQ=255)
                                                                                                                                     cTGGCG‑AAA‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACATTAttt                   >  1:3323226/1‑151 (MQ=255)
                                                                                                                                     cTGGCG‑AAA‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACATTAttt                   >  1:2401475/1‑151 (MQ=255)
                                                                                                                                      tGGCG‑AAA‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACATTAtttt                  >  1:3078107/1‑151 (MQ=255)
                                                                                                                                         cG‑AAA‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACATTATTTTCat               >  1:2872754/1‑151 (MQ=255)
                                                                                                                                          g‑AAA‑GGTTTAAAAAAAAA‑T‑CTTGG‑TTTTG‑ATTAAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACATTATTTTCata              <  1:1353383/151‑1 (MQ=255)
                                                                                                                                          g‑AAA‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACATTATTTTCata              >  1:2103825/1‑151 (MQ=255)
                                                                                                                                            aaa‑GCTTTGAAAGAAAA‑G‑CTTTG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACATTATTTTCatat             >  1:2519581/1‑151 (MQ=255)
                                                                                                                                            aaa‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACATTATTTTCatat             >  1:2980998/1‑151 (MQ=255)
                                                                                                                                             aa‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACATTATTTTCATATg            >  1:3406850/1‑151 (MQ=255)
                                                                                                                                             aa‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACATTATTTTCATATg            >  1:2748716/1‑151 (MQ=255)
                                                                                                                                             aa‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACATTATTTTCATATg            >  1:3400225/1‑151 (MQ=255)
                                                                                                                                              a‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACATTATTTTCATATGt           >  1:797606/1‑151 (MQ=255)
                                                                                                                                                     aaaagaaag‑G‑CTTGG‑CATTG‑AGTAAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACATTATTTTCATATGTACACCg     <  1:2207505/150‑1 (MQ=255)
                                                                                                                                                      aaagaaaA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACATTATTTTCATATGTACACCgg    >  1:1968966/1‑151 (MQ=255)
                                                                                                                                                        agaaaA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACATTATTTTCATATGTACACCGGtt  >  1:3421445/1‑151 (MQ=255)
                                                                                                                                                        agaaaA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACATTATTTTCATATGTACACCGGtt  >  1:2510316/1‑151 (MQ=255)
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ACAACTTCCGCCGGAAGATTTCTGTGAACTGTGTAAAGATATAAGTAAAATTGCCGAAATATTAGGAA‑GAGAGTTTCACTATGCAGGTTGAAAAGATATCCATTATCGGGCTTGG‑ACTTATCGGCGGGTCGCTGGCG‑AAA‑GCTTTGAAAGAAAA‑G‑CTTGG‑CATTG‑AGTCAATAACCGCCGTTGACATCAATGAGAAAAGTCTGAGCCAGGCTCTTAAAGAGGG‑TTTTATAAAAGAAGGTTTTACCGAACTTAACGAATCGGTATATAATTCTGACATTATTTTCATATGTACACCGGTT  >  CP002416/2884188‑2884486

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑